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Archive > Volume 25 Issue 3 > 2018,25(3):270-274. DOI:10.3872/j.issn.1007-385X.2018.03.010 Prev Next
Basic Research
Effect of Tim-4 on invasion and migration of SiHa cervical cancer cell and its un- derlying mechanism
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Abstract:
Objective: To investigate the effect of Tim-4 on invasion and migration of cervical cancer cells and its underlying mechanisms. Methods：The expression levels of Tim-4 in cervical cancer cell lines Siha, Hela and cervical epithelial immortalized cell line H8 were detected by Real-time PCR. The Tim-4 lentiviral vector was transfected into Siha cell line. The over-expression of Tim-4 in Siha cell line was detected by fluorescence microscopy. The effects of Tim-4 on the invasion and migration of cervical cancer cell line were detected by Transwell and scratches methods. The changes of MMP2, MMP9, E-cadherin and N-cadherin in Siha cells were detected by Western blotting. Results：The expression of Tim-4 was higher in Siha and Hela cell lines compared to that in the H8 cell line. The Siha cell line burdening Tim-4 lentiviral vector was successfully constructed. Over-expression of Tim-4 significantly inhibited the migration and invasion of cervical cancer cell line, and affected the expression of MMP2, MMP9, N-cadherin and E-cadherin. Conclusion：Over-expression of Tim-4 promotes the invasion and migration by regulating the EMT transformation in cervical cell carcinoma.
Keywords:
Project Supported:
Project surpported by the Nature Science Fund of Shandong Province （No. ZR2015HM031，ZR2014HM042）, and the Medical and Health Science and Technology Development Plan of Shandong Province (No. 2016WSA03022)
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