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Archive > Volume 25 Issue 4 > 2018,25(4):346-350. DOI:10.3872/j.issn.1007-385X.2018.04.005 Prev Next
Clinical Research
Expression of zinc transporter 1 gene in brain glioma tissues and its effects on pro- liferation, migration and invasion of glioma U87 cell line
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Abstract:
Objective: To detect the expression of zinc transporter 1 (ZnT1) gene in glioma tissue, and to explore its effect on the prolif- eration, migration and invasion of U87 cells. Methods: From October 2015 to January 2017, 20 patients with glioma, who received no chemoradiotherapy before operation, were collected from Department of Neurosurgery, the First Affiliated Hospital of China Medical University. The protein and mRNA content of ZnT1 in glioma tissues and adjacent tissues were detected by Western blotting and Real- time PCR, respectively. ZnT1 and si-ZnT1 plasmids were transfected into glioma U87 cell line respectively to construct ZnT1 over-ex- pression U87 cell line and ZnT1 knockdown U87 cell line. The effects of ZnT1 on proliferation, migration and invasion of U87 cells were detected by MTT and transwell assay. Results: Both mRNA and protein expressions of ZnT1 in glioma tissues was significantly higher than those in adjacent tissues (all P<0.05). U87 cell lines with ZnT1 over-expression and knockdown were successfully construct- ed. Compared with the control group and empty plasmid control group, the proliferation (0.54±0.01 vs 0.45±0.04, 0.43±0.03, P<0.01), invasion and migration (all P<0.05) of U87 cells with ZnT1 over-expression were significantly increased at 12 h after transfection; how- ever, the proliferation (0.37±0.03 vs 0.45±0.01, 0.44±0.03, P<0.01), invasion and migration (all P<0.05) of U87 cells with ZnT1 knock- down were decreased significantly. Conclusion: ZnT1 was highly expressed in glioma tissues, and promoted the proliferation, migra- tion and invasion of glioma U87 cells.
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Project Supported:
Project supported by the Overseas Returnees Research Foundation of Ministry of Education (No. [2013]1792); the Natural Science Foundation of Liaoning Province (No.2015020460); the Chinese Postdoctoral Science Foundation (No.2016M590240); the Science And Tech- nology Project of Shenyang City (No.17-230-9-13); and the Scientific Research Foundation of the First Affiliated Hospital of China Medical University (No.FSFH201722)
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