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Archive > Volume 25 Issue 4 > 2018,25(4):351-356. DOI:10.3872/j.issn.1007-385X.2018.04.006 Prev Next
Clinical Research
Expression of FOXD1 in glioma tissues and its correlation with prognosis of patients
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Abstract:
Objective: To evaluate the expression level of FOXD1 in glioma tissues of different grades, and to investigate the correla- tion between the expression of FOXD1 and the prognosis of glioma patients. Methods: The tumor tissues were collected from 40 glio- ma patients, who received surgical treatment in the neurosurgery department of the First Hospital of China Medical University from September 2014 to February 2015; Seven non-tumor tissues obtained from patients underwent internal decompression for traumatic brain injury were used as controls. The FOXD1 expression in glioma and non-tumor brain tissues was analyzed by qRT-PCR and IHC, and the correlations between clinical pathological features of glioma patients and FOXD1 expression level were analyzed. Furthermore, the Kaplan-Meier method was used to analyze the relationship between FOXD1 expression and survival time of patients. In addition, the expression of FOXD1 in glioma tissues and its relationship with patients’prognosis were confirmed by the data from GEO (GSE4290, GSE2223) and Rembrandt database. Results: qRT-PCR showed that the FOXD1 mRNA expression in glioma tissues of WHO grade IV was significantly higher than that of non-tumor brain tissues and glioma tissues of WHO grade II (P<0.01). German im- munohistochemical score (GIS) was used to evaluate the immunohistochemical staining intensity, and the relationship between FOXD1 expression and clinical pathological features was analyzed. The results showed that FOXD1 in glioma tissues was related to WHO pha- thological grade level (χ 2 =11.73，P<0.01). There was statistically significant difference between the survival time of FOXD1 high ex- pression group and FOXD1 low expression group (P=0.043). The data from GEO data base (GSE4290, GSE2223) and Rembrandt data- sets showed that glioma tissues have a higher FOXD1 mRNA expression level than normal brain tissues, and the elevated expression of FOXD1 mRNA was negatively associated with the survival time of glioma patients. Conclusion: FOXD1 was highly expressed in glio- ma tissues, and the expression level of FOXD1 was increased as the pathological grade increases. The elevated expression of FOXD1 was related with the poor survival of glioma patients.
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Project Supported:
Project supported by the Science and Technology Plan Projects of Liaoning Province (No.2012225014)
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