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Archive > Volume 25 Issue 5 > 2018,25(5):480-484. DOI:10.3872/j.issn.1007-385X.2018.05.007 Prev Next
Basic Research
Effects of siRNA interfering TWSG1 expression on proliferation and apoptosis of gastric cancer MGC-803 cells
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Abstract:
[Abstract] Objective: To study the effects of twisted gastrulation protein homolog 1 (TWSG1) gene on proliferation and apoptosis of gastric cancer MGC-803 cells. Methods: Three siRNAs for TWSG1 gene were designed.The MGC-803 cells at logarithmic phase were divided into blank control group, negative control group (siRNA-NC), siRNA1 interference group, siRNA2 interference group and siRNA3 interference group by transfecting with relevant vectors. The mRNA and protein expressions of TWSG1 in each group were identified by qPCR and Western blotting, respectively; and the stable cell line with highest interference efficiency was screened.The proliferation of cells in each group was detected by CCK-8 assay, and the apoptosis of three groups was detected by flow cytometry. Results:The results of qPCR and Western blotting showed siRNA1 exhibited highest interference efficiency. Compared with the blank control group and the negative control group, the expression of TWSG1 in siRNA interference cell group was lower (P<0.05), the cell proliferation significantly increased (P<0.05), and apoptosis significantly reduced (P<0.05). Conclusion: siRNA interfering TWSG1 expression in MGC-803 cells can promote cell proliferation, inhibit cell apoptosis.
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Project Supported:
Project supported by the Guangdong Science and Technology Program (No. 2015A010107005),and the Guangdong Provincial Natural Science Foundation (No.2017A030310022)
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