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Archive > Volume 25 Issue 6 > 2018,25(6):574-581. DOI:10.3872/j.issn.1007-385X.2018.06.005 Prev Next
Basic Research
Effect of co-culture with amniotic epithelial cells on biological characteristics of amniotic mesenchymal stem cells
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Abstract:
[Abstract] Objective: The aim of this study was to investigate the effect of co-culture with AEC (amniotic epithelial cell) on the biological characteristics of AMSC (amniotic mesenchymal stem cell), and to investigate the roles of SDF-1/CXCR4 axis in the homing and migration of AMSC. Methods: AMSC and AEC were isolated from human amnion, and then cultured, amplified and identified, respectively.The AMSC were divided into three groups: AEC co-cultured group, serum-free cultured group and serum cultured group. After culture for 24 h, 48 h, and 72 h, the proliferation viability of AMSC was measured by CCK-8 assay and trypan blue staining; the expression of CXCR4 mRNA was analyzed by flow cytometry and Real-time RT-PCR, and the migration ability of AMSC in vitro was observed by migration assay. Results: Cell viability (48 h and 72 h) and survival rate in the co-culture and serum groups were higher than those in the serum-free group (all P<0.05). The mRNA and protein expressions of CXCR4 in AMSC of the co-culture and serum-free groups were significantly higher than those of the serum group (P<0.05). The migration ability of AMSC in the co-culture and serumfree groups, which increase with the SDF-1 (stromal cell derived factor-1) concentration gradient, were higher than that in the serum group (P<0.05). Conclusion: AMSC co-cultured with AEC still have the basic biological characteristics of MSC, and showed good growth activity. Co-culture with AEC can up-regulate CXCR4 on AMSC surfaces and enhance the migration ability of AMSC in vitro.
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Project Supported:
Projects supported by the National Natural Science Foundation of China (No.31260232), the Scientific Research Project of Medical and Health Institutions of Yunnan Province (No. 31260232), and the Applied Basic Research Joint Foundation of Yunnan Provincial Science and Technology Department and Kunming Medical University（No. 2017FE468-030）
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