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Archive > Volume 25 Issue 11 > 2018,25(11):1105-1118. DOI:10.3872/j.issn.1007-385X.2018.11.005 Prev Next
Basic Research
miR-138-5p regulates invasion and migration of gastric cancer cell SGC-7901 via targeting TCF3
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Abstract:
Objective: To explore the target relationship between miR-138-5p and TCF3, and its effect on invasion and migration of human grastric cancer SGC-7901 cells. Methods: After being transfected with miR-138-5p mimic, the relative mRNA expression of miR-138-5p and TCF3 in SGC-7901 cells was detected by qRT-PCR. Bioinformatics methods were used to predict the targeted matching relationship between miR-138-5p and TCF3 gene, which was then verified by the luciferase reporter gene system. After transfecting normal gastric cancer cells and TCF3 high expression gastric cancer cells with miR-138-5p mimic, Western blotting was performed to detect the protein expressions of TCF3, N-cadherin, Vimentin, Slug and E-cadherin; Transwell assay and scratch assay were used to detect the invasion and migration ability of gastric cancer cells, respectively. Results: Over-expression of miR-138-5p inhibited the expression of TCF3 mRNA. Bioinformatics software predicted that miR-138-5p and TCF3 mRNA had targeted binding areas.miR-138-5p mimics reduced the luciferase activity of TCF3 wild-type plasmids, without affecting the luciferase activity of TCF3 mutant-type plasmids. miR-138-5p inhibited the expression of TCF3 protein, and attenuated the promotion effect of TCF3 over-expression on the invasion and migration of SGC-7901 cells, in the meanwhile, down-regulated the protein expressions of N-cadherin, Vimentin and Slug proteins, and up-regulated the protein expression of E-cadherin. Conclusion: miR-138-5p directly regulates the expression of TCF3 and affects the invasion and migration of gastric cancer SGC-7901 cells.
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Project Supported:
Project surpported by the Science and Technology Project of Henan Province（No. 162102310329）
Clc Number:
