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Archive > Volume 25 Issue 11 > 2018,25(11):1125-1130. DOI:10.3872/j.issn.1007-385X.2018.11.007 Prev Next
Basic Research
Effect of Caveolin-1 on activation of epidermal growth factor receptor 2 in gastric cancer cells
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Abstract:
Objective: To investigate the effect of Caveolin-1(Cav-1) protein on the malignant biological behaviors of NCI-N87 gastric cancer cells, and further to analyze the possible molecular mechanisms. Methods: NCI-N87 cell line that stably expressing Cav-1 was constructed; MTT assay, colony formation assay and wound healing assay were used to examine the effect of Cav-1 on the malignant biological behaviors of gastric cancer cells; Western bloting was used to detect the effect of Cav-1 protein on Her-2 activation as well as the ERK and Akt signaling pathway under the induction with EGF ligand. Results: Over-expression of Cav-1 significantly inhibited the proliferation and migration of NCI-N87 cells. With the stimulation of EGF ligand, Cav-1 over-expression significantly inhibited the tyrosine phosphorylation level of Her-2 and the ratio of P-ERK/ERK as well as P-AKT/AKT. Conclusion: The over-expression of Cav-1 could significantly inhibit Her-2 tyrosine phosphorylation, and may inhibit the proliferation and migration of NCI-N87 cells via the down-stream MAPK and PI3K/Akt signaling pathway.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81071846), the WU Jieping Medical Foundation (No. 320.6750.14063), and the Key Project of Medical Science Research in Hebei Province（No. 20170698）
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