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Archive > Volume 25 Issue 12 > 2018,25(12):1237-1243. DOI:10.3872/j.issn.1007-385X.2018.12.005 Prev Next
Basic Research
Effect of miR-141-3p targeting TGF- β2 on malignant biological behaviors of human prostatic cancer C4-2B cells
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Abstract:
Objective: To investigate the relationship between miR-141-3p and transforming growth factorβ2 (TGF-β2), and its effects on the malignant biological behaviors of human prostate cancer cell line C4-2B. Methods: After the transfection of miR-141-3p mimic,the mRNA expression of miR-141-3p and TGF-β2 in C4-2B cells was detected by qRT-PCR. Bioinformatics method validated the relationship between miR-141-3p and TGF-β2. miR-141-3p mimic alone or with TGF-β2 over-expression vector was transfected into C4-2B cells, and then Western blotting was used to detect the expression of TGF- β2 protein in C4-2B cells, Hochest33258 staining was used to detect cell apoptosis, and Transwell assay was used to detect the invasion ability of cells in each group. Results: After the transfection of C4-2B cells with miR-141-3p mimic, the level of miR-141-3p increased significantly, and the level of TGF- β2 mRNA decreased significantly (all P<0.01). The activity of luciferase was significantly reduced after the co-transfection with miR-141-3p mimic and wild type report plasmid (P<0.01); However, the activity of luciferase was not obviously changed after co-transfection with miR-141-3p mimic and mutant type report plasmid (P>0.05). After co-transfection with miR-141-3p mimic and pc-TGF-β2, the proliferation of C4-2B cells decreased significantly, the number of apoptotic cells increased significantly, and the cell invasion ability decreased significantly (all P<0.01). Conclusion: miR-141-3p inhibits the proliferation and invasion of human prostate cancer C4-2B cells and induces cell apoptosis by targeting TGF-β2.
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Project Supported:
Project supported by the Department of Science and Technology of Sichuan Province (No.2014JY0187)
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