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Archive > Volume 25 Issue 12 > 2018,25(12):1264-1269. DOI:10.3872/j.issn.1007-385X.2018.12.009 Prev Next
Basic Research
Inhibitory effect of recombinant oncolytic adenovirus on luciferase-labeled and non-labeled human lung cancerA549 cells
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Abstract:
Objective: To investigate the inhibitory effect of recombinant oncolytic adenovirus Ad-Apoptin-hTERTp-E1A (ATV) on luciferase-labeled human lung cancer cells (A549-luc) and human lung cancer A549 cells, and to compare the differences in the inhibitory effect on two cell lines. Methods: ATV was used to infect A549-luc cells and A549 cells respectively. WST-1 and crystal violet staining were used to determine the difference in the inhibitory effect of ATV. Hoechst and Annexin V-FITC/PI staining were used to verify the inhibition mode of ATV. Results: WST-1 and crystal violet staining showed that ATV had significant inhibitory effect on both A549-luc and A549 cells (P<0.05). ATV showed significant inhibitory effect on both cells at 24, 48 and 72 h (P<0.05 or P<0.01), and reached the peak at 72 h; ATV at concentrations of 1, 10 and 100 MOI all showed inhibitory effect on both cells, and reached the peak at 100 MOI.Hoechst staining showed that A549-luc cells and A549 cells infected with ATV showed typical nuclear fragmentation and marginal set.The results of Annexin V-FITC/PI Flow cytometry showed that ATV infection resulted in apoptosis of A549-luc and A549 cells, which was in a time-dependent manner and reached the peak at 72 h(P<0.05 or P<0.01). Conclusion: Insertion of luciferase didn’t significant-ly change the inhibitory effect and inhibitory mode of ATV on A549-luc cells. ATV exerted its in vitro inhibitory effect on A549-luc and A549 cells by inducing cell apoptosis.
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Project Supported:
Project supported by the National Key Research and Development Program of China on Prevention and Treatment of Important New Outbreaks of Disease (No. 2016YFC1200900), the Major Science and Technology Program of Changchun City (No. 16ss11), and the National Major Scientific and Technological Special Project for“ Significant New Drugs Development”(No.2018ZX09301053-004-001)
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