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Archive > Volume 26 Issue 2 > 2019,26(2):146-151. DOI:10.3872/j.issn.1007-385X.2019.02.002 Prev Next
Basic Research
GSDME influences sensitivity of breast cancer MCF-7 cells to paclitaxel by regulating cell pyroptosis
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Abstract:
[Abstract] Objective: To investigate whether GSDME affects the sensitivity of breast cancer MCF-7 cells to paclitaxel (PTX) by regulating cell pyroptosis. Methods: GSDME was knocked-down in MCF-7 cells by RNA interference technique. CCK-8 assay, flow cytometry,lactate dehydrogenase (LDH) release method and Wb were respectively used to detect cell proliferation, pyroptotic rate, LDH release, GSDME-N-terminal protein and cleaved-caspase-3 protein levels in PTX-treated MCF-7 cells before and after GSDME knockdown.Results: Compared with the control group, the pyroptotic rate, LDH release, GSDME-N-terminal protein and cleaved-caspase-3 protein levels in the PTX-treatment group significantly increased (all P<0.01). Compared with the si-NC group, the PTX-sensitivity of si-GSDME group decreased, and the pyroptotic rate, LDH release and GSDME-N-terminal protein all significantly decreased (all P<0.01). Conclusion: Knock-down of GSDME in MCF-7 cells significantly inhibited cell pyroptosis and reduced drug sensitivity of MCF-7 cells to PTX.
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Project Supported:
Project supported by the Key Research Program of Higher Education in Henan Province（No. 19A320055）
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