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Archive > Volume 26 Issue 2 > 2019,26(2):196-199. DOI:10.3872/j.issn.1007-385X.2019.02.009 Prev Next
Basic Research
Triptolide inhibits activity of human cervical microvascular endothelial cells
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Abstract:
[Abstract] Objective: To investigate the inhibitory effect of Triptolide on vasculogenesis of human cervical microvascular endothelial cells, and to explore the mechanism. Methods: Human cervical microvascular endothelial cells (HCerMECs) were used as research subject,and treated with different concentrations of Triptolide (0, 5, 10, 20 and 40 ng/ml) in vitro. The effect of Triptolide on cell proliferation was determined by CCK-8, the cell migration ability was detected by Transwell assay while the expression of vascular endothelial growth factor (VEGF) was examined by western blotting. Results: Triptolide inhibited the proliferation and migration of HCerMECs in a dose-dependent manner (P<0.05). In addition, Triptolide could inhibit the expression of VEGF in HCerMECs in a concentration-dependent manner. Conclusions: Triptolide could inhibit the proliferation and migration activity of HCerMECs which is related with the suppression of VEGF expression.
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