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Archive > Volume 26 Issue 5 > 2019,26(5):512-517. DOI:10.3872/j.issn.1007-385X.2019.05.005 Prev Next
Basic Research
Effect of WTAP gene knockdown on malignant biological behaviors of lung adenocarcinoma A549 cells
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Abstract:
[Abstract] Objective: To investigate the effects of Wilms’tumor 1-associating protein (WTAP) on proliferation, migration and invasion of human lung adenocarcinoma A549 cells. Methods: Human lung adenocarcinoma cell line A549 and HEK293T cells were chosen for this study. Two sets of shWTAP interference sequences were designed to construct lentiviral vector plasmid. Human lung adenocarcinoma A549 cells were infected after packaging lentivirus in HEK293T cells, and the control group was transfected with 277 empty vector plasmid. The mRNA and protein expression levels of WTAP in A549 cells were detected by qPCR and WB. Changes in proliferation,migration and invasion of A549 cells were detected by BrdU assay, cell scratch healing assay and Transwell assay, respectively.Results: Two plasmids, shWTAP-1 and shWTAP-2, were successfully constructed. Compared with the control group, the mRNA and protein expression levels of WTAP were significantly down-regulated in A549 cells with WTAP knockdown (both P<0.05), and the proliferation,migration and invasion ability of cells were significantly decreased (all P<0.05). Conclusion: Knockdown of WTAP significantly inhibited the proliferation, migration and invasion of human lung adenocarcinoma A549 cells. The expression of WTAP gene is associated with the occurrence and development of lung adenocarcinoma. WTAP may be a potential target for the diagnosis and treatment of lung adenocarcinoma.
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Project Supported:
Project supported by the National Key Laboratory Open Research Project (No.2018094), the Tianjin Municipal Health and Family Planning Commission and Tianjin Municipal Administration of Traditional Chinese Medicine Key Projects (No. 2017057), the Graduate Innovation Funding Project of Hebei Province (No.CXZZSS20181), and the Tianjin Health andWellness Committe Project (No.2014kZ053)
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