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Archive > Volume 26 Issue 11 > 2019,26(11):1214-1221. DOI:10.3872/j.issn.1007-385X.2019.11.006 Prev Next
Clinical Research
Expression and clinical significance of ADNP in bladder urothelial carcinoma tissues
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Abstract:
Objective：To analyze the expression and clinic significance of activity-dependent neuroprotective protein (ADNP) in bladder urothelial carcinoma. Methods: A total of 28 pairs of bladder cancer tissues and corresponding adjacent normal tissuesthat surgically resected at the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University from June 1, 2019 to July 15,2019 were collected for this study. The mRNA expression of ADNP in 20 pairs of tissue samples was detected by qPCR, and the protein expressionin the other 8 pairs was detected by WB. Mean while, the clinicopathological data of patients with bladder urothelial carcinoma treated in our hospital from January 1, 2005 to December 31, 2007 were retrospectively analyzed; and the expression of ADNP in the corresponding paraffin tumor sections were determined with immunohistochemical staining, and normal bladder tissue sections from patients who underwent surgery for other bladder diseases during the same period were collected for comparison. Chi-square test was used to analyze the correlation between ADNP expression and different clinicopathological features, Kaplan-Meier method was used for survival analysis, and Cox risk regression model was used for univariate and multivariate analysis of prognostic factors. Results:The transcriptional and translational levels ofADNPin cancer tissues were higher than those in adjacent normal tissues (all P<0.05), and the expression level of ADNP was correlated with the histological grade, clinical stages and survival status of patients with bladder cancer (P<0.05). Of all the 221 patients included in the study, 32 patients lost to follow-up,and patients with high ADNP expression had poor prognosis (5-year OS: 49.5% vs 78.6%, P<0.01; 5-year PFS: 40.0% vs 72.2%, P<0.01; 10-year OS: 26.6% vs 58.6%, P<0.01; 10-year PFS: 25.3% vs 47.9%, P<0.01). Cox univariate regression analysis showed that ADNP expression was closely related to the prognosis of bladder cancer (P<0.05); and Cox multivariate regression analysisalso showed that ADNP expression (95% CI: 1.300-2.905, P=0.001) was an independent risk factor for the prognosis of bladder cancer. Conclusion: The expression level of ADNP in bladder cancertissuesis significantly higher than that in non-tumor bladder tissues. Histological grade and clinical stageare correlated with the expression level of ADNP. The prognosis of bladder cancer patients with low ADNP expression is relatively better compared to patients with high ADNP expression, andADNP is expected to be a candidate target for specific treatment of bladder cancer.
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Project Supported:
Project supported by Key Research and Development Program from the Science and Technology Department of Hunan Province（No. 2018SK2120）
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