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Salidroside regulates DC through TLR4 to increase the lethality of T cells to lung cancer 3LL cells
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Abstract:
Objective：To investigate the effect of salidroside (SAL) on the phenotype of dendritic cells (DCs) and the antitumor ability of cytotoxic T lymphocytes (CTL). Methods：Lewis lung cancer cell line 3LL, wild type (WT) C57BL/6 mice and TLR4-/- C57BL/6 mice were chosen for this study. Mice bone marrow derived DC precursor cells were obtained to differentiate into immature DCs,which were harvested on the sixth day of culture. CD11c+ DCs were obtained by magnetic beads screening, and further divided into PBS group, SAL group and lipopolysaccharide (LPS) group. After being cultured for 48 h, the effects of SAL on surface molecules and phagocytosis of DCs as well as the efffect of TLR4 pathway on the killing effect of T cells were detected by Fow cytometry. Results：Compared with PBS group, expressions of DC surface molecules CD80, CD86 and MHC Ⅱ significantly increased (all P<0.05), phago‐cytosis significantly decreased (P<0.05), and TLR4 expression level significantly increased (P<0.01) in SAL group; Compared with WT group, after being treated with SAL or LPS, the expressions of DC surface molecules CD80, CD86 and MHC Ⅱ decreased signifi‐cantly in TLR4-/- group (all P<0.05); Compared with PBS group, the activated CTLin SAL group exhibited a significantly elevated killing effect against lung cancer 3LL cells (P<0.05). Conclusion：SAL can induce DC maturation by regulating TLR4, thus improving the kill‐ing ability of T cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81560668,No.81860719), the Natural Science Foundation of Tibet Autonomous Region (No.XZ2017ZRG-59, No.XZ2018ZRG-72), and the Tibetan School Youth Teacher Innovation Support Project（No.QCZ2016-36）
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