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Archive > Volume 27 Issue 4 > 2020,27(4):377-384. DOI:10.3872/j.issn.1007-385X.2020.04.006 Prev Next
Basic Research
Effect of alantolactone on malignant biological behaviors of human osteosarcoma 143B cells
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Abstract:
Objective: To investigate the effect of alantolactone (ALT) on proliferation, migration, invasion and apoptosis of human osteosarcoma 143B cells and the underlying mechanism. Methods: Osteosarcoma 143B cells were treated with different concentrations of ALT (0, 4, 6, 8, 10 μmol/L). Then, the cell proliferation ability was detected by crystal violet staining and MTT assay, cell migration was determined by Wound-healing test, cell invasion was analyzed by Transwell assay and cell apoptosis rate was detected by Hoechst33258 staining. The mRNA and protein expressions of E-cadherin, N-cadherin, caspase-3, cleaved caspase-3 (c-caspase-3),poly ADP-ribose polymerase (PARP) and cleaved PARP (c-PARP) in 143B cells were detected by qPCR and Western blotting (WB),respectively. TCF/LEF (T cell lymphocyte factor/lymphoid enhancer factor) transcriptional activity was examined with Luciferase reporter gene assay. The mRNA and protein expressions of β -catenin as well as MMP-7 and c-Myc were detected by qPCR and WB,respectively. Results: ALT inhibited proliferation, migration and invasion of osteosarcoma 143B cells and promoted apoptosis (P<0.05 or P<0.01). After the treatment with ALT at 8, 10 μmol/L, the mRNA and protein expressions of E-cadherin and PARP, as well as the protein expressions of c-caspase-3 and c-PARP were up-regulated, while the mRNA and protein expressions of N-cadherin were downregulated (P<0.05 or P<0.01); At the same time, the TCF/LEF transcriptional activity and the mRNAand protein expressions of β-catenin,MMP-7 and c-Myc were significantly down-regulated (P<0.05 or P<0.01). Conclusion: ALT may inhibit the proliferation, migration and invasion and promote cell apoptosis possibly through suppressing Wnt/β-catenin signaling pathway in osteosarcoma 143B cells.
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Project Supported:
Project supported by the Scientific and Technological Research Program of Chongqing (No. cstc2017jcyjAX0039)
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