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Archive > Volume 27 Issue 4 > 2020,27(4):403-409. DOI:10.3872/j.issn.1007-385X.2020.04.010 Prev Next
Clinical Research
Histone demethylase JMJD3 promotes the stemness of diffuse large B cell lymphoma cells
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Abstract:
Objective: To investigate the effect of histone demethylase JMJD3 (jumonji domain-containing protein 3) on the stemness of diffuse large B-cell lymphoma (DLBCL) cells. Methods: The relationship between the expression of JMJD3 and the overall survival of DLBCL patients was analyzed using the clinical data of DLBCL patients in TCGA database. The control plasmid (pCMV) and JMJD3 expression plasmid (pCMV-JMJD3) were transfected into DLBCL cells ofABC and GCB subtype via lipofectamine transfection.Then, the mRNA levels of JMJD3, ALDH1, OCT4 and SOX2 were detected by RT-PCR and qPCR; the activity of ALDH1 enzyme was detected by Flow cytometry; the protein expressions of OCT4 and SOX2 were detected by Western blotting. Gene enrichment in DLBCL patients with high JMJD3 expression was analyzed by gene set enrichment analysis (GSEA). Results: The result of prognosis analysis showed that high expression of JMJD3 was related with poor prognosis in DLBCL patients (P<0.05); however, multivariate analysis showed that the expression of JMJD3 was not the independent factor affecting the prognosis of DLBCL patients (all P>0.05).The expression of JMJD3 was remarkably increased in DLBCL cells transfected with pCMV-JMJD3, which led to significantly increased mRNA level and enzyme activity of ALDH1 as well as up-regulated mRNA and protein expressions of OCT4 and SOX2 (P<0.05 or P<0.01). GSEA analysis showed that enrichment of Wnt/β -catenin signaling pathway related gene set was observed in DLBCL patients with high JMJD3 expression (P<0.05). Conclusion: JMJD3 promotes the stemness of DLBCL cells, which may be a potential therapeutic target for DLBCL patients.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81673012, No.81602496), and the Natural Science Foundation of Tianjin (No.18JCQNJC 11100)
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