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Archive > Volume 27 Issue 4 > 2020,27(4):410-415. DOI:10.3872/j.issn.1007-385X.2020.04.011 Prev Next
Clinical Research
Diagnostic value of combined detection of multiple serum cytokines for gastric adenocarcinoma
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Abstract:
Objective: To explore the clinical significance of multiple serum cytokines in early diagnosis and progression assessment of gastric adenocarcinoma. Methods: Peripheral blood samples of 85 healthy subjects (healthy control group) and 81 patients with pathologically confirmed gastric adenocarcinoma (gastric cancer group) were collected from November 2017 to February 2018 at Shanxi Cancer Hospital.Serum levels of 17 cytokines (including IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-15, IL-17A, TNF-α, TNF-β, GM-CSF, G-CSF,IFN-γ, IP-10, MCP-1 and VEGF-A) were measured byAimPlex multiplex assay technology. Their diagnostic values were analyzed by receiver operating characteristic (ROC) curve. Results: Serum levels of IL-10, IL-8, IL-6, IP-10, MCP-1, VEGF-A and IL-12p70 were significantly higher in gastric cancer patients than those in healthy controls (all P<0.01). There were significantly increased levels of IL-8, IL-6 and VEGF-Ain advanced-stage gastric cancer (stage I/II) group over early-stage gastric cancer (stage III/IV) group (all P<0.01). AUC (areas under the curve) of IL-8, IL-6, IL-10, IP-10, MCP-1, IL-12p70 and VEGF-A for distinguishing early-stage gastric cancer patients from healthy controls was 0.98, 0.92, 0.89, 0.84, 0.76, 0.74 and 0.58, respectively. The diagnostic sensitivity of IL-8, IL-6 and IL-10 was 97.4%, 89.5% and 97.4%, respectively, and the specificity was 87.1%, 85.9%and 77.6%, respectively. TheAUC of IL-8, IL-6 and VEGF-Afor distinguishing advanced-stage gastric cancer patients from early-stage gastric cancer patients was 0.82, 0.72 and 0.69, respectively. Thediagnosticsensitivity of IL-8, IL-6 and VEGF-A was 83.7%, 60.5% and 41.9%, respectively, and the specificity was 71.1%, 76.3%and 92.1%, respectively.Conclusion: The combined detection of serumIL-8, IL-6 and IL-10 may be a potential approach for early screening of gastric adenocarcinoma,which can also be used to assess the progression of gastric adenocarcinoma.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81272696), the Special Foundation for Talents of Shanxi Province (No.201705D211021), and the Key Research and Development Program of Shanxi Province (No.201903D321027)
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