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Archive > Volume 27 Issue 5 > 2020,27(5):496-500. DOI:10.3872/j.issn.1007-385X.2020.05.004 Prev Next
Basic Research
miRNA-325-3p reduces the radio-sensitivity of nasopharyngeal carcinoma CNE1 cells by down-regulating the expression of cytokeratin 13
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Abstract:
Objective：To investigate the effect of miRNA-325-3p and its target gene cytokeratin 13 (CK13) on the radio-sensitivity of nasopharyngeal carcinoma cell line CNE1. Methods：The potential target gene of miRNA-325-3p was predicted by three databases:miRBase, Targetscan and microcosm, and verified by Double luciferase activity assay. QPCR was used to detect the expression levels of miRNA-325-3p and its target gene in nasopharyngeal carcinoma cell line CNE1 under different radiation doses; To verify the changes in radio-sensitivity of nasopharyngeal carcinoma cells, colony formation assay, Flow cytometery and MTT were used to observe the clone formation, apoptosis and cell viability of CNE1 cells after overexpression of miRNA-325-3p and knockdown of CK13 under different radiation doses, respectively. Results：CK13 was confirmed as a potential target gene of miRNA-325-3p. After radiotherapy, the expression level of miRNA-325-3p in CNE1 cell was significantly increased, while the expression level of CK13 was decreased (all P<0.05); up-regulation of miRNA-325-3p expression and silence of CK13 gene increased cell survival rate (upregulation of miRNA-325-3p: [60.14±3.55]% vs [19.23±3.42]%, t=14.37, P<0.01; silence of CK13: [76.15±5.13]% vs [28.53±3.68]%, t=13.06, P<0.01)and colony formation rate, and decreased apoptosis rate (upregulation of miRNA-325-3p: [27.95±2.67]% vs [51.68±3.47]%, t=9.39,P<0.01; silence of CK13: [20.31±2.62]% vs [38.14±3.83]%, t=6.66, P<0.01). Conclusion：miRNA-325-3p can reduce the sensitivity of nasopharyngeal carcinoma cell line CNE1 to radiotherapy by down-regulating the target gene CK13.
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Project Supported:
Project supported by Joint Program of Applied Basic Research of Yunnan Provincial Department of Science and Technology-Kunming Medical University [No. 2018FE001(-173)]
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