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Archive > Volume 27 Issue 7 > 2020,27(7):764-769. DOI:10.3872/j.issn.1007-385X.2020.07.007 Prev Next
Clinical Research
TGF- β induced lncRNA linc01503 promotes proliferation, invasion and EMT process of esophageal squamous cell carcinoma cells
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Abstract:
Objective: To investigate the expression of lncRNA linc01503 in esophageal squamous cell carcinoma (ESCC) tissues and cell lines, as well as its effect on the proliferation, invasion and epithelial-mesenchymal transition (EMT) of ESCC cells. Methods: A total of 119 pairs of tumor tissues and corresponding para-cancerous tissues of ESCC patients were obtained from the Fourth Affiliated Hospital of Hebei Medical University between Jan. 2012 and Dec. 2014. The expression of linc01503 in ESCC tissues and cell lines (Kyse150, Kyse170, Eca109, TE1 and TE13) was detected by qPCR. The ESCC cells were transfected with pGPU6-shRNA-linc01503 or treated with TGF-β. The expressions of EMT related genes before and after transfection as well as linc01503 expression before and after TGF-β treatment were detected with qPCR. MTS and Transwell assay were performed to assess the effect of linc01503 on proliferation and invasion of ESCC cells. Results: The expression of linc01503 was significantly elevated in ESCC tissues and cell lines (all P<0.05). High expression of linc01503 was correlatedwith lymph node metastasis, depth of infiltration, TNM stage and the survival of ESCC patients (all P<0.05). Treatment with TGF-β promoted EMT of ESCC cells and induced a significant up-regulation of linc01503 expression. Knockdown of linc01503 significantly inhibited proliferation and invasion ability of ESCC cells; Meanwhile, the low expression of linc01503 increased the expression of E-cadherin but decreased the expressions of N-cadherin and vimentin (all P<0.05).Conclusion: lncRNA linc01503, as one of the downstream effect genes of TGF- β , promotes the proliferation, invasion and EMT process of ESCCcells.
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Project Supported:
Project supported by the Natural Science Foundation of Hebei Province (No. H2019206664), the Key Project of Medical Science Research in Hebei Province (No. 20170698), and the Talent Engineering Training Project of Hebei Province (No. 201901035)
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