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Archive > Volume 28 Issue 2 > 2021,28(2):121-127. DOI:10.3872/j.issn.1007-385x.2021.02.004 Prev Next
Basic Research
Effect of over-expression of vasohibin-1 on malignant biological behavior of human colorectal cancer cells
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Abstract:
Objective: To investigate the effects of over-expressing VASH1 on the malignant biological behaviors of human colorectal cancer cells.Methods: Lentivirus was packaged and transfected into human colorectal cancer cells SW680 and SW620 to construct an over-expressed VASH1 cell line, the untransfected cells were used as control. qPCR experiment and WB experiment were used to detect the over-expression effect of VASH1. The effects of VASH1 over-expressed on microangiogenesis, proliferation, colony formation and migration of colorectal cancer cells were detected respectively by tubule formation, CCK-8 assay, soft agar assay, Transwell assay and Wound healing assay in vitro. In addition, tumor growth and lung metastasis were detected in NOD-SCID mice subcutaneously injected with VASH1-overexpressing SW620 cells.Results: Successfully constructed SW480 and SW620 cells over-expressing VASH1. Compared with the control group, the abilities of microangiogenesis, proliferation, colony forming and migration were significantly reduced in colorectal cancer cells over-expressing VASH1 (P<0.05) in vitro. The abilities of subcutaneous tumor growth and lung metastasis of colorectal cancer cells over-expressing VASH1 were also significantly reduced (P<0.05) in vivo.Conclusion: Over-expression of VASH1 can suppress the malignant biological behaviors of human colorectal cancer cells.
Keywords:
Project Supported:
Project supported by the Tianjin Health and Family Planning Commission Grant (No. 2014KZ053, No. 2017057), the Key Projects of Tianjin Science and Technology Support Plan (No. 19YFZCSY00420), the Open Research Foundation of State Key Laboratory of Medicinal Chemical Biology (No. 2018094), the Major Special Projects of Science and Technology for Prevention and Control of Major Diseases in Tianjin (No. 18ZXDBSY00040), and the Tianjin Union Medical Center Grant (No. 2016YJ035)
Clc Number:
R735.3; R730.54
