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Archive > Volume 28 Issue 5 > 2021,28(5):460-468. DOI:10.3872/j.issn.1007-385X.2021.05.006 Prev Next
Basic Research
circ_0091579/miR-330-3p/RNF126 axis affects the proliferation, invasion and apoptosis of colorectal cancer LoVo cells
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Abstract:
[Abstract] Objective: To identify the effect of circRNA circ_0091579 on the proliferation, apoptosis and invasion of colorectal cancer (CRC) cells by functioning as a molecular sponge of miR-330-3p and mediating ring finger protein 126 (RNF126). Methods: Sixty pairs of cancer tissue and adjacent normal tissue of CRC patients receiving surgical treatment in the First Affiliated Hospital of Dali University from February 2019 to May 2020 were collected. CRC LoVo cells with over-expression or knockdown of circ_0091579 and miR-330-3p were constructed. The expression of circ_0091579, miR-330-3p and RNF126 in CRC tissue and cell lines (HCT116, SW620, CW-2 and LoVo cells) was detected by qPCR. The proliferation, invasion and apoptosis were measured by MTT assay, Transwell assay and Flow cytometry, respectively. The targeting relationship between circ_0091579 and miR-330-3p, miR-330-3p and RNF126 was predicted by bioinformatics tool and verified by Dual luciferase reporter assay and RNA immunoprecipitation experiments. Results: In CRC tissues and cell lines, circ_0091579 and RNF126 were both highly expressed, and miR-330-3p was lowly expressed (all P＜ 0.05). Knockdown of circ_0091579 could inhibit the proliferation and invasion, but promote the apoptosis of CRC cells ( P＜ 0.05); however, this effect was reversed by miR-330-3p-inhibitor. Over-expression of miR-330-3p could suppress the ability of proliferation and invasion, but enhance the apoptosis of LoVo cells ( P＜0.05), and this effect was rescued after the participation of RNF126. There is a targeting relationship among circ_0091579, miR-330-3p and RNF126. Conclusion: circ_0091579 promotes the proliferation and invasion, and inhibits the apoptosis of LoVo cells via miR-330-3p/RNF126 axis.
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Project Supported:
Project supported by the Scientific Research Fund of the Education Department of Yunnan Province (No. 2019J0767)
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