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Archive > Volume 28 Issue 5 > 2021,28(5):469-476. DOI:10.3872/j.issn.1007-385X.2021.05.007 Prev Next
Basic Research
circ_0072088 promotes the malignant biological behaviors of non-small cell lung cancer cells by regulating miR-545-3p/STAT3 axis
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Abstract:
[Abstract] Objective: To explore the biological function and mechanism of circular RNA (circRNA) 0072088 in non-small cell lung cancer (NSCLC) cells. Method: GSE101684 data set was downloaded from Gene Expression Omnibus (GEO), and the differentially expressed genes (DEGs) were screened with GEO2R analysis. The expression of circ_0072088 in NSCLC cell lines (H165， H358， H460， H226 and A549 cells) was detected using qPCR. CCK-8 assay and Transwell chamber assays were utilized to assess cell proliferation, migration and invasion. CircInteractome and TargetScan database were used to predict the targeting relationship between circ_0072088 and miR-545-3p as well as between miR-545-3p and STAT3, which were then verified by Dual luciferase reporter gene experiment and RNA binding protein immunoprecipitation (RIP) experiment. Results: The expression of circ_0072088 in NSCLC cell lines was significantly up-regulated (P <0.05). Over-expression of circ_0072088 promoted the proliferation, invasion and migration of NSCLC cells (P <0.05); knockdown of circ_ 0072088 inhibited the proliferation, invasion and migration of NSCLC cells (P <0.05). MiR-545-3p is the downstream target of circ_0072088 and can be sponged by circ_0072088; STAT3 is the target gene of miR-545-3p, which can be indirectly and positively regulated by circ_0072088. Conclusion: Circ_0072088 promotes the proliferation and metastasis of NSCLC cells by regulating the miR-545-3p /STAT3 axis.
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Project Supported:
Project supported by the Science and Technology Research and Development Plan Project of Zhangjiakou City (No.1911021D-3)
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