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Archive > Volume 28 Issue 5 > 2021,28(5):477-481. DOI:10.3872/j.issn.1007-385X.2021.05.008 Prev Next
Basic Research
Enterococcus faecalis lipoteichoic acid activates TLR2 to inhibit the proliferation,invasion and migration of pancreatic ductal cancer BxPC-3 cells
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Abstract:
[Abstract] Objective: To investigate the effect of Enterococcus faecalis lipoteichoic acid (LTA) on the proliferation, invasion and migration of pancreatic ductal adenocarcinoma (PDA) cell line BxPC-3 and its possible mechanism. Methods: BxPC-3 cells were treated with 0, 5, 10 and 50 μg/ml LTA; the 0 μg/ml group was set as the blank control group, while the other groups were used as the experimental groups. CCK-8 method was used to detect the effect of LTA on the proliferation of BxPC-3 cells. BxPC-3 cells were treated with 50 μg/ml LTA for 48 h, and the invasion and migration abilities of BxPC-3 cells were detected by the Scratch test and Transwell chamber test, respectively. WB assay was used to detect the effect of LTA on protein expression of TLR2, P38, p-P38, NF-κB and p-NF-κB in BxPC-3 cells. Results: LTA inhibited the proliferation of BxPC-3 cells, and the inhibitory effect increased with time and concentration. Compared with the 0 μg/ml group, LTA at 50 μg/ml exhibited the most significant inhibitory effect on proliferation of BxPC-3 cells after 48 h (P<0.01); thus, the subsequent experimental groups were treated with 50 μg/ml LTA for 48 h. Compared with the 0 μg/ml group, the number of invaded cells and the migration rate of BxPC-3 cells in the 50 μg/ml group were significantly reduced (all P<0.01), and the protein expression levels of TLR2, p-P38 and p-NF-κB were significantly increased (all P<0.01). Conclusion: Enterococcus faecalis LTA can inhibit the proliferation, invasion and migration of pancreatic cancer BxPC-3 cells. The mechanism may be related to the activation of TLR2 by LTA to promote phosphorylation of P38 and NF-κB.
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