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Archive > Volume 28 Issue 5 > 2021,28(5):482-488. DOI:10.3872/j.issn.1007-385X.2021.05.009 Prev Next
Clinical Research
Expression of Claudin-2 in human esophageal squamous cell carcinoma tissues and its effect on the malignant biological behaviors of KYSE-450 cells
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Abstract:
[Abstract] Objective: To analyze the expression of Claudin-2 in human esophageal squamous cell carcinoma (ESCC) tissues and its relationship with clinicopathological characteristics and 5-year survival rate of ESCC patients, and to explore its effect on proliferation, migration and invasion of ESCC KYSE450 cells. Methods: A total of 52 cases of tumor tissues and 20 cases of corresponding para-cancerous tissues that surgically removed from ESCC patients who were primarily treated in Henan Cancer Hospital from 2010 to 2013 were collected for this study. Claudin-2 expression was analyzed by Immunohistochemistry and WB assay, and its relationship with clinicopathological characteristics and 5-year survival rate of ESCC patients was further investigated. The expression of Claudin-2 in ESCC cells (KYSE450, KYSE150, KYSE510 and KYSE140) and human immortalized esophageal epithelial SHEE cells was detected by WB assay. The Claudin-2 shRNA lentiviral vector was established and transfected into KYSE450 cells to establish a Claudin-2 knockdown cell line. Furthermore, the effects of knockdown of Claudin-2 on the proliferation, migration and invasion of KYSE450 cells were tested by clone formation experiment, cell scratch experiment and Transwell experiment. Results: The positive rate of Claudin-2 in ESCC tissues was significantly higher than that in adjacent tissues (P<0.05). The expression of Claudin-2 in ESCC tissues was related to lymph node metastasis (P<0.05). The 5-year survival rate of Claudin-2 positive patients was significantly lower than that of negative patients (P<0.05). The KYSE450 cell line with Claudin-2 knockdown was successfully constructed. The number of clone formation, wound healing rate and number of invaded cells in the sh-Claudin-2 group were significantly lower than those in the sh-NT group and the control group (all P<0.05). Conclusion: The expression level of Claudin-2 in ESCC tissues is higher than that of the tumor-adjacent tissues, and is negatively related with 5-year survival rate of ESCC patients. Claudin-2 can promote the proliferation, migration and invasion of ESCC cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81602637), and the Key R&D and Promotion Projects of Henan Province (No.02102310089)
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