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Archive > Volume 28 Issue 5 > 2021,28(5):495-503. DOI:10.3872/j.issn.1007-385X.2021.05.011 Prev Next
Clinical Research
The expression of minichromosome maintenance protein 3 in brain glioma and its clinical significances
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Abstract:
[Abstract] Objective: Analyze the expression, clinical significance and possible biological processes of minichromosome maintenance protein 3 (MCM3) in brain gliomas, and explore its relationship with glioma immunity. Methods: The databases GEPIA and Oncomine were searched online to obtain the expression of MCM3 in glioma tissues. The CGGA database was used to analyze the relationship between MCM3 expression and clinicopathological characteristics of gliomas online.At the same time, 24 tumor specimens of patients with glioma who underwent surgical treatment in the Department of Neurosurgery of Shanxi Provincial People's Hospital from January 2019 to March 2020 and 8 non-tumor control specimens were collected, and used immunohistochemical SP method to detect the expression of MCM3 to verify the bioinformatics analysis results. Kaplan-Meier survival curve was used to evaluate the effect of MCM3 on the prognosis of glioma in the TCGA and CGGA databases.The significant related genes of MCM3 were obtained through Linkedomic database, STRING database and Cytoscape software. Use the DAVID database to perform GO and KEGG analysis of MCM3 and its significant related genes to explore the biological processes they may participate in.Finally, explore the relationship between MCM3 expression and glioma immune infiltrating cells in the TIMER database. Results: Comprehensive bioinformatics analysis and clinical data verification showed that MCM3 was highly expressed in glioma tissues relative to normal tissues (P=0.024), and its expression level gradually increased with the pathological grade (P=0.001).Survival analysis showed that high expression of MCM3 was related to the poor prognosis of glioma (P<0.05).GO and KEGG analysis of MCM3 and its significant related genes showed that these genes are mainly enriched in cell cycle, DNA replication and regulation of DNA damage repair.TIMER database showed that in the glioma cohort, MCM3 was correlated with a variety of immune infiltrating cells (P<0.05). Conclusions: MCM3 is highly expressed in gliomas and is related to poor prognosis, which may be related to the cell cycle, DNA replication, regulation of DNA damage repair and immune microenvironment of glioma cells. MCM3 can promote the progression of glioma, and can be used as a prognostic indicator and potential therapeutic target for patients with glioma.
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Project Supported:
Project supported by the Shanxi Provincial Applied Basic Research Program General Youth Fund Project (No. 201901D211478), the Science and Technology Innovation Project of Higher Education Institutions in Shanxi Province(No. 2020L0200), and the Shanxi Province Key R&D Project (Social Development) (No. 201903D321045)
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