Mobile website
Archive > Volume 28 Issue 5 > 2021,28(5):504-510. DOI:10.3872/j.issn.1007-385X.2021.05.012 Prev Next
Clinical Research
Clinical significance of BRD-containing protein members in hepatocellular carcinoma progression
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To reveal the correlation between BRD (bromodomain)-containing protein family and hepatocellular carcinoma (HCC) from different perspectives such as transcripts, protein levels, gene mutations, protein interactions, corresponding signaling pathways and functional enrichment by searching and mining HCC-related data in multiple tumor public databases, and to explore the potential of BRDs as biological markers for tumor progression and prognosis prediction of HCC. Methods: The mRNA expression profile of all BRD-containing proteins and clinical information of HCC patients were obtained from UALCAN database, and their correlation was subsequently analyzed. The relationship between mRNA expression of BRDs and prognosis of HCC patients was analyzed by using TCGA database. The immunohistochemical results of BRDs in HCC tissues and normal liver tissues were obtained from The Human Protein Atlas, and the differential expression was compared. The STRING database was used to analyze the PPI (protein-protein interaction) network of BRDs, and the CYTOSCAPE software was used for the KEGG and GO analyses of the interacting molecules. Results: All the 7 BRD family members were highly expressed in HCC tissues (P<0.01) and were positively correlated with tumor grade and clinical stage in HCC patients (P<0.05); moreover, HCC patients with low BRD8 and BRD9 expression had better prognosis (P<0.01). In addition, BRD interacting proteins were mainly involved in histone acetylation modification and were highly enriched in HCC signaling pathways. The expressions of BRD1, BRD3, BRD4, BRD7, BRD8 and BRD9 in HCC patients with TP53 mutation were significantly higher than those in patients without TP53 mutation (P<0.05). Conclusion: The BRD-containing proteins might be used as potential biomarkers for tumor grade, clinical stage, and prognosis prediction of HCC patients.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No.81871229)
Clc Number:
