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The expression of minichromosome maintenance protein 3 in brain glioma and its clinical significances
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Abstract:
Objective: To explore the effect of miR-1207-5p on the proliferation, migration and invasion of breast cancer T47D stem cells and its possible mechanism. Methods: Breast cancer T47D stem cells were induced and enriched with IGF-1, EGF and bFGF and cultured into spheroids. The stem cells were separated by Flow cytometry, and molecular markers of stem cells were detected by WB. qPCR was used to detect the expression level of miR-1207-5p in stem cells, and Dual luciferase reporter gene assay was used to analyze the targeting relationship between miR-1207-5p and LIMD1. CCK-8, Transwell and Cell scratch test were used to detect the proliferation, migration and invasion ability of T47D stem cells. WB was used to detect the expression level of LIMD1 protein in stem cells. Results: The isolated stem cells could form cell spheres, and the volume of the cell spheres increased with the increase of culture days. The expressions of stem cell molecular markers ALDH1, ESA and OCT4 were significantly higher than those of the parental T47D cells (P<0.05 or P<0.01 ), and miR-1207-5p was highly expressed in stem cells (P<0.01). Overexpression of miR-1207-5p significantly promoted the proliferation, migration and invasion of T47D stem cells (all P<0.01), and knockdown of miR-1207-5p significantly inhibited the proliferation, migration and invasion of T47D stem cell (all P<0.01). miR-1207-5p targetedly downregulated the expression of LIMD1 (P<0.01), by which it promoted the proliferation, migration and invasion of breast cancer T47D stem cells (P<0.05 or P<0.01). Conclusion: miR-1207-5p promotes the proliferation, migration and invasion of breast cancer T47D stem cells by targeted downregulation of the expression of LIMD1.
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Project Supported:
Project supported by the Chen Xiaoping Science and Technology Development Foundation Project (No. CXPJJH11900002-038)
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