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LINC00519 regulates the proliferation, apoptosis, migration and invasion of gastric cancer cells through the miR-876-3p/HMGA1 axis
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Abstract:
Objective: To investigate the effect of long intergene non-coding RNA 00519 (LINC00519) on the proliferation, apoptosis, migration and invasion of gastric cancer HGC-27 cells by regulating the miR-876-3p / high mobility group protein A1 (HMGA1) axis. Methods: The expression levels of LINC00519 in gastric cancer HGC-27 cells and gastric mucosal epithelial GES-1 cells were detected by qPCR. HGC-27 cells were divided into si-NC, si-LINC00519, si-LINC00519+anti-miR-NC, and si-LINC00519 + anti-miR[1]876-3p groups according to transfection treatment. Colony formation test was applied to detect cell cloning ability; Flow cytometry was used to detect apoptosis and cell cycle distribution; Transwell assay was selected to measure cell migration and invasion. Dual luciferase reporter gene assay and qPCR were applied to confirm the interaction between LINC00519 and miR-876-3p as well as between miR-876-3p and HMGA1. Results: The expression of LINC00519 in HGC-27 cells was significantly higher than that in GES[1] 1 cells (P <0.05). After siRNA transfection, the expression level of LINC00519 in HGC-27 cells of the si-LINC00519 group was significantly lower than that of the si-NC group (t=47.294, P<0.01). Compared with the si-NC group, the number of HGC-27 cell clones, the number of migrated and invaded cells, and proportion of S-phase cells in the si-LINC00519 group were significantly decreased (all P<0.01), while the apoptosis rate and the proportion of G0/G1 phase cells were significantly increased (all P <0.01). Compared with the si-LINC00519+anti-miR-NC group, the number of HGC-27 cell clones, the number of migrated and invaded cells, proportion of S-phase cells were significantly increased (all P<0.01), while the apoptosis rate and the proportion of G0/G1 phase cells in the si-LINC00519 + anti-miR-876-3p group were significantly decreased (all P<0.01), LINC00519 could target and negatively regulate miR-876-3p expression. miR-876-3p could target and negatively regulate HMGA1 expression. Conclusion: Knocking down LINC00519 could inhibit the proliferation, migration and invasion of gastric cancer HGC-27 cells and induce apoptosis by regulating the miR-876-3p/HMGA1 axis.
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