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Clinical Research
circ_0072088 promotes the malignant biological behaviors of thyroid cancer IHH4 cells by regulating the miR-532-3p/WEE1 axis
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Abstract:
Objective: To explore the effect of circ_0072088 on the malignant biological behaviors of thyroid cancer IHH4 cells and its possible mechanism. Method: From April 2015 to March 2019, the thyroid cancer tissues and corresponding adjacent tissues from 48 patients who were diagnosed as thyroid cancer and underwent resection without any previous treatment in the Affiliated Hospital of Qinghai University were collected. The differentially expressed circRNAs in papillary thyroid cancer were identified by GEO database (GSE93522). The expression pattern of circ_0072088 was determined using quantitative real-time polymerase chain reaction (qRT-PCR) in thyroid cancer samples and cell lines. CCK-8 and Transwell assays were performed to assess the effects of circ_0072088 and miR-532-3p on proliferation, migration, and invasion of thyroid cancer IHH4 cells. Bioinformatics analysis and dual-luciferase reporter assays were used to explore the correlation between circ_0072088 and miR-532-3p as well as between miR-532-3p and WEE1 gene in thyroid cancer cells. Results: circ_0072088 expression was upregulated in thyroid cancer tissues and cell lines ( P<0.05 or P<0.01). Overexpression of circ_0072088 promoted the proliferation, migration, and invasion of thyroid cancer cells (P<0.05 or P<0.01), while transfection of miR-532-3p mimics weakened this effect (P<0.05 or P<0.01). Mechanistically, circ_0072088 could bind to miR-532-3p. Besides, WEE1 was proved to be the downstream target gene of miR-532-3p. Conclusion: Circ_0072088 promotes the proliferation, migration and invasion of IHH4 cells through regulating WEE1/miR-532-3p axis.
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