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Expression of miR-203a-3p in pancreatic cancer tissues and cells and its effect on proliferation, migration and invasion of BxPC-3 cells
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Abstract:
Objective: To investigate the effects of miR-203a-3p on the proliferation, migration and invasion ability of pancreatic cancer BxPC-3 cells. Methods: The Cancer Genome Atlas (TCGA) database was used to screen the differentially expressed miRNAs between pancreatic cancer tissues and paracancerous tissues, and to analyze the survival rate and clinical stage of pancreatic cancer patients with high or low miRNA expression; TarBase was used for the cancer related GO function and KEGG pathway analysis of the miRNAs, DIANATools, miRDB and TargetScan websites were used to predict the target genes of miR-203a-3p. miR-203a-3p mimic and NC mimic, miR-203a-3p inhibitor and NC inhibitor were transfected into BxPC-3 cells. The expression levels of miR-203a-3p, miR-192-5p and miR-451a in normal pancreatic epithelial HPNE cells and pancreatic cancer cells were detected by qPCR. The proliferation, migration, invasion and colony formation abilities of BxPC-3 cells were detected by CCK-8, Transwell chamber assay and Colony formation assay, respectively. Results: A total of 18 differentially expressed miRNAs in pancreatic cancer tissues were screened out by TCGA database, among which miR-203a[1]3p, miR-192-5p and miR-451a were species-conservative and significantly correlated with clinical cancer stage, cell cycle and survival rate of pancreatic cancer patients (all P<0.05); Bioinformatics tool predicted that PPM1A might be the candidate target gene of miR[1] 203a-3p and could interact with multiple genes. miR-203a-3p, miR-192-5p and miR-451a were highly expressed in BxPC-3 and Aspc[1] cells (all P<0.001). In miR-203a-3p mimic group, the expression level of miR-203a-3p and the proliferation, migration and invasion ability of BxPC-3 cells were significantly increased (all P<0.01); however, in miR-203a-3p inhibitor group, the expression level of miR-203a-3p and the ability of cell proliferation, migration and invasion were significantly decreased (all P<0.01). Conclusion: miR-203a-3p is highly expressed in pancreatic cancer tissues and cells, and its expression is related to the survival and clinical stage of patients. miR-203a-3p may regulate the proliferation, migration and invasion of BxPC-3 cells.
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Project Supported:
Project supported by the Youth Fund of National Natural Science Foundation of China (No. 81201281), the Government-Funded Clinical Medicine Talents Training Project of Hebei Province (2020) (No. [2020]397), the Science and Technology Research and Development Program from Tangshan of Hebei Province (No. 19130204C), the Basic Scientific Research Business Expenses of Universities in Hebei Province (No. jqn2020005), the Key Research and Development Project in Hebei Province (No. 213777115D), and the Innovation Project for College Students of North China University of Science and Technology (No. X2020083)
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