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miR-206 inhibits the proliferation and invasion of estrogen-induced ER-α36-positive gastric cancer BGC-823 cells by targeting CDK14
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Abstract:
Objective: To explore the effects of miR-206 on the proliferation and invasion of ER-α36-positive gastric cancer (GC) BGC-823 cells induced by estrogen and its related mechanisms. Methods: ER- α36-positive BGC-823 cells were stimulated with estradiol (E2) at different concentrations (1, 10 and 100 pmol/L), then, the expression level of miR-206 was detected by qPCR, the proliferation and invasion were respectively determined by MTT and Transwell assays, and the expression of cyclin-dependent kinase 14 (CDK14) protein was detected by WB. After transfecting miR-206 mimic, miR-NC, pcDNA-CDK14 or pcDNA-vector into ER-α36 positive BGC-823 cells and stimulating them with 100 pmol/L E2, the proliferation and invasion ability of the cells were respectively detected by MTT assay and Transwell assays, and the expression of CDK14 was detected by WB assay. The targeting relationship between miR-206 and CDK14 was verified by Dual luciferase reporter gene assay. Results: E2 significantly decreased the expression level of miR-206 (P<0.05 or P<0.01), enhanced the proliferation and invasion (P<0.05 or P<0.01), and up-regulated the expression level of CDK14 （P<0.01) in ER-α36-positive BGC-823 cells. Overexpression of miR-206 could significantly reduce the proliferation and invasion ability of ER-α36-positive BGC-823 cells induced by E2 (all P<0.01). miR-206 negatively regulated the expression of CDK14 by directly binding to the 3'-UTR of CDK14 mRNA, thereby inhibiting the proliferation and invasion of ER- α -positive BGC-823 cells (all P<0.01). Conclusion: miR-206 inhibits the proliferation and invasion of estrogen-induced ER- α36-positive GC cells by targeting CDK14.
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Project Supported:
Project supported by the Natural Science Foundation of Anhui Province (No. 1908085QH333)
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