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Effects of 1, 25(OH)2D3 regulating ERK pathway on the biological behaviors of esophageal squamous cell carcinoma cells
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Abstract:
Objective: To investigate the effects of 1,25(OH)2D3 (1,25-dihydroxy vitamin D3) on the proliferation, migration and cell cycle of esophageal squamous cell carcinoma (ESCC) cells and its mechanism. Methods: Human ESCC cells (TE-1, KYSE30, TE-11 and KYSE510) were treated with different concentrations of 1,25(OH)2D3, and the proliferation was detected by CCK-8. TE-11 and KYSE30 cells were treated with 1,25(OH)2D3 at concentrations of 0, 0.1, 0.15, and 0.2 μmol/L, respectively. Scratch healing test was used to detect cell migration ability, Flow cytometry was used to detect cell cycle distribution, and WB was used to detect the protein expressions of cyclin D1, P27, ERK and p-ERK. Results: 1,25(OH)2D3 significantly inhibited the proliferation of TE-11, KYSE30 cells in a concentration- and time-dependent manner (P<0.05 or P<0.01). As compared with control group, TE-11 and KYSE30 cells that treated with 0.1 and 0.2 μmol/L 1,25(OH)2D3 for 48 h exhibited significantly inhibited migration ability (P<0.05 or P<0.01)，increased cell population at G0/G1 phase (P<0.05 or P<0.01)，decreased protein expressions of cyclin D1 and p-ERK (P<0.05 or P<0.01)，and increased expression of P27 protein (P<0.05 or P<0.01)；however, there was no significant difference in total ERK level. Conclusion: 1,25(OH)2D3 can significantly inhibit the proliferation, migration and block the cell cycle of ESCC cells, which may be through the regulation of ERK signal pathway.
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Project Supported:
Project supported by the Sichuan Science and Technology Innovation Seedling Project (No.2020104), the Science and Technology Program of Sichuan Province (No.2018SZ0377, No.2018JY0219), and the Nanchong City-School Cooperative Scientific Research Special Fund (No.18SXHZ0208, No.18SXHZ0366, No.18SXHZ0381)
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