Mobile website
Clinical Research
Expression of miR-17-5p in gastrointestinal stromal tumor tissues and its effect on proliferation and apoptosis of GIST882 cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the expression of miR-17-5p in gastrointestinal stromal tumor (GIST) tissues and its effect on proliferation and apoptosis of GIST882 cells. Methods: Twenty pairs of GIST tissues and corresponding paratumoral tissues form patients who underwent gastrointestinal surgery in the Fourth Affiliated Hospital of Guangxi Medical University from May 2019 to May 2020 were selected for this study; at the same time, GIST882 cells and human intestinal epithelial cells (HIECs) were also collected for this study. The KIT gene mutations in GIST tissue samples were detected by fluorescence PCR-capillary electrophoresis sequencing. The miR-17-5p mimics and pc-KIT plasmids were transfected into GIST882 cells, respectively. The targeting relationship between miR-17-5p and KIT was verified by Dual-luciferase reporter gene assay. The mRNA and protein expressions of miR-17-5p and KIT in GIST tissues and GIST882 cells were detected by qPCR and WB, respectively; and the proliferation, apoptosis and cell cycle progression of the cells were detected by CCK-8 and Flow cytometry. Results: KIT gene mutations occurred in 15 GIST patients (15/20). Compared with paratumoral tissues, miR-17-5p expression was significantly decreased while KIT mRNA expression was significantly increased in GIST tissues (all P<0.01); compared with HIEC cells, miR-17-5p expression was significantly decreased while mRNA and protein expressions of KIT were significantly increased in GIST882 cells (all P<0.01). Overexpression of miR-17-5p significantly decreased the proliferation ability and increased the apoptosis rate of GIST882 cells (P<0.01 or P<0.05), increased the proportion of cells in sub-G1 and S phases (P<0.05), and decreased the expression level of KIT protein (P<0.01). Dual-luciferase reporter gene assay confirmed that KIT is a downstream target gene of miR-17-5p. Simultaneous overexpression of miR-17-5p and KIT did not produce significant effects on the proliferation, cell cycle progression and apoptotic levels of GIST882 cells. Conclusion: Overexpression of miR-17-5p can significantly inhibit the proliferation and induce apoptosis of GIST882 cells and downregulate the expression of KIT protein. miR-17-5p may be a potential target in the treatment of GIST.
Keywords:
Project Supported:
Project supported by the Research Program of Health Commission of Guangxi Zhuang Autonomous Region (No. Z2016176, No. Z20190443)
Clc Number:
