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Archive > Volume 28 Issue 8 > 2021,28(8):810-817. DOI:10.3872/j.issn.1007-385X.2021.08.007 Prev Next
Clinical Research
Expression of forkhead box protein D3 in gastric cardia adenocarcinoma tissues and its effect on biological behaviors of SGC-7901 cells
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Abstract:
Objective: To investigate the expression of forkhead box protein D3 (FOXD3) in gastric cardia adenocarcinoma (GCA) and its effect on the biological behaviors of SGC-7901 cells. Methods: A total of 49 pairs of GCA tissues and corresponding para[1]cancerous tissues that surgically resected from June 2014 to December 2016 were selected from the Biological Specimen Library of the Fourth Hospital of Hebei Medical University. qRT-PCR was used to detect the expression of FOXD3 in GCA tissues and corresponding para-cancerous tissues, as well as in gastric cancer cell lines（BGC-823, SGC-7901, HGC-27, MGC-803, and NCI-N87）. pc-DNA3.1-FOXD3 or pc-DNA3.1 was transfected into SGC-7901 cells. Cell proliferation assay, clone formation assay, scratch healing assay, and Transwell chamber invasion assay were used to detected the effect of FOXD3 overexpression on the the proliferation, clone formation,migration, and invasion of SGC-7901 cells. The mRNA and protein expressions of epithelial-mesenchymal transition (EMT) related molecules before and after the transfection were detected by qRT-PCR and WB. Cell cycle changes before and after transfection were detected by Flow cytometry. Results: The expression of FOXD3 mRNA in GCA tissues was significantly downregulated, and it's expression was closely correlated with clinical stage and lymph node metastasis of GCA patients. The expression of FOXD3 in gastric cancer cell lines was lower than that in normal cell lines (all P<0.01). FOXD3 overexpression significantly inhibited the proliferation,clone formation, migration and invasion of SGC-7901 cells in vitro (all P<0.01). FOXD3 overexpression could up-regulate the expression of E-cadherin and down-regulate the expressions of N-cadherin, β -catenin and vimentin in SGC-7901 cells (all P<0.01). Overexpression of FOXD3 could arrest the cell cycle in the G0/G1 phase (P<0.01). Conclusion: The expression of FOXD3 in GCA tissues is significantly downregulated, and its overexpression can inhibit the biological behaviors of gastric cancer cells. FOXD3 can be used as a tumor suppressor gene to provide new ideas for tumor therapy.
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Project Supported:
Project supported by the Natural Science Foundation of Hebei Province (No. H2019206664
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