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Archive > Volume 28 Issue 9 > 2021,28(9):869-876. DOI:10.3872/j.issn.1007-385X.2021.09.002 Prev Next
Basic Research
Characteristics and function of T cells in tumor-draining lymph nodes of colorectal cancer
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Abstract:
Objective: To investigate the immunological characteristics and the anti-tumor potential of T cells in the tumor-draining lymph node (TDLN) of colorectal cancer (CRC). Methods: The clinical data and lymph node specimens of 33 CRC patients who were treated in the Affiliated Hospital of Guizhou Medical University from December 2018 to January 2021 were retrospectively collected for this study. Paired TDLN and non-tumor-draining lymph nodes (NTDLN) were collected from CRC patients by using Dye tracer. Flow cytometry was used to examine the immune cell subsets and functional phenotypic differences in TDLN and NTDLN that prepared as single cell suspension. ELISPOT (enzyme-linked immunoSPOT) method was employed to compare the proportion of tumor-reactive T cells in TDLN and NTDLN. The spatial distribution of immune cells in the TDLN and NTDLN was evaluated by multiplexed immunohistochemistry (mIHC). The TDLN-T cells were amplified ex vivo to evaluate the T cell subsets and phenotypic changes as well as tumor immune response capabilities. Results: Compared with NTDLN, there were higher proportions of tumor[1]reactive T cells and regulatory T cells (Tregs), but lower proportion of monocytic myeloid-derived suppressor cells (Mo-MDSCs) in TDLN (P<0.01 or P<0.05 ); in addition, both the activation markers (ICOS, CD28) and suppression markers (PD-1, TIGIT) were elevated (P<0.05 or P<0.01 ). mIHC results showed that Tregs were mainly distributed in the cortex, and follicular helper T (Tfh) cells were located in the germinal center. After the TDLN-T cells were expanded ex vivo, CD8+ T cells were the predominant phenotype （P<0.01), and the expression of ICOS and CD28 and the proportion of tumor-reactive T cells were increased (P<0.05 or P<0.01 ).T cells in colorectal cancer TDLN are activated and highly expresses immune suppression markers. Ex vivo expansion can enhance the activation of TDLN-T cells and increase the proportion of tumor-reactive T cells.
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Project Supported:
Project supported by the Department of Science and Technology of Guizhou Province (No. [2019]2788), and the National Natural Science Foundation of China, Guizhou Medical University Cultivation Project (No.19NSP045)
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