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Archive > Volume 28 Issue 9 > 2021,28(9):885-892. DOI:10.3872/j.issn.1007-385X.2021.09.004 Prev Next
Basic Research
miR-153-3p regulates proliferation, invasion and migration of gastric cancer SGC7901 cells via targeting FZD3
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Abstract:
Objective: To investigate the effect of miR-153-3p on the proliferation, invasion and migration of gastric cancer SGC7901 cells and its molecular mechanism. Methods: A total of 60 pairs of cancer tissues and corresponding para-cancerous tissues of gastric cancer patients who were treated in the General Hospital of Ningxia Medical University during May 2018 and June 2020 were collected for this study; in addition, human gastric cancer cell lines (NCI-N87, AGS, SNU-5, SGC7901) and normal gastric epithelial GES-1 cells were also collected. The expression level of miR-153-3p in gastric cancer tissues and cells was detected by qRT-PCR. miR-153-3p mimics and mimic control were transfected into SGC7901 cells, respectively. The effects of miR-153-3p overexpression on proliferation, apoptosis, invasion and migration of gastric cancer SGC7901 cells were respectively determined using CCK-8, Clone formation assay, Flow cytometry, TUNEL, Transwell and Wound-healing assay. SGC7901 cell transplanted xenograft tumor model in nude mice was established to explore the effect of miR-153-3p on tumor growth. The targeting relationship between miR-153-3p and FZD3 was predicted and verified by bioinformatics database and Dual-luciferase reporter gene assay, respectively. The effects of miR-153-3p on expression of FZD3 protein and Wnt/β-catenin pathway related proteins were determined by Western blotting. Results: The expression level of miR-153-3p in gastric cancer tissues and cells was significantly lower than that in para-cancerous tissues and gastric epithelial cells, with the lowest expression in SGC7901 cells (all P<0.01). Up-regulation of miR-153-3p significantly inhibited the proliferation, invasion and migration abilities of SGC7901 cells, and increased the rate of apoptotic cells (all P<0.01). In addition, up-regulation of miR-153-3p significantly promoted the expression of E-cadherin but significantly suppressed the expression of N-cadherin, matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9) (all P<0.01). In vivo experiments showed that intravenous injection of miR-153-3p mimics significantly reduced tumor size and Ki-67 expression, but significantly increased the expression of P57 in tumor tissues (all P<0.01). The mechanism analysis showed that miR-153-3p could bind to the 3'UTR region of FZD3 mRNA, and up-regulation of miR-153-3p inhibited the expression of FZD3 and increased the levels of β -catenin, TCF-4 and cyclin D1 (all P<0.01). Conclusion: MiR-153-3p regulates the proliferation, invasion and migration of gastric cancer SGC7901 cells via targeting FZD3 gene and regulating Wnt/β-catenin signaling pathway.
Keywords:
Project Supported:
Project supported by the Natural Science Foundation of Ningxia (No.2019AAC03200, No.2019AAC03225)
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