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Archive > Volume 28 Issue 9 > 2021,28(9):893-899. DOI:10.3872/j.issn.1007-385X.2021.09.005 Prev Next
Basic Research
circ_0001821 regulates the proliferation, apoptosis, migration and invasion of cutaneous squamous cell carcinoma A431 cells by targeting miR-203
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Abstract:
Objective: To explore the mechanism of circ_0001821 regulating the proliferation, apoptosis, migration and invasion of cutaneous squamous cell carcinoma (CSCC) A431 cells by targeting miR-203. Methods: The cancer and para-cancerous tissues of 39 patients with CSCC treated in the Second Affiliated Hospital of Hainan Medical University from February 2018 to August 2019, as well as the human CSCC cell line A431, were collected for this study. The qPCR method was used to detect the expression level of circ_0001821 in CSCC tissues and para-cancerous tissues. Using liposome transfection technology, si-circ_0001821, si-NC, miR-203 mimic, miR-NC, si-circ_0001821+anti-miR-NC and si-circ_0001821+anti-miR-203 were transfected into A431 cells, respectively. qPCR was used to detect the expression of circ_0001821 and miR-203 in the transfected cells. MTT method, Flow cytometry and Transwell chamber assay were used to detect the proliferation, apoptosis, migration and invasion of transfected A431 cells. WB was used to detect the expression of proteins related to proliferation, apoptosis, migration and invasion. Circinteractome database was utilized to predict the binding site between circ_0001821 and miR-203, which was further validated by Dual-luciferase reporter gene assay. Results: Compared with para-cancerous tissues, the expression level of circ_0001821 in CSCC tissues was significantly increased (P<0.01). Transfection of si-circ_0001821 could significantly reduce the expression of circ_0001821 (P<0.01), increase the apoptosis rate (P<0.01), and reduce the proliferation, migration and invasion abilities of A431 cells (all P<0.01). Dual-luciferase report gene assay confirmed that circ_0001821 could targetedly bind with miR-203. Transfection of miR-203 mimics could significantly reduce the proliferation, migration and invasion abilities but increase the apoptosis rate of A431 cells (all P<0.01). . Co-transfection of si-circ_0001821 and anti-miR-203 could significantly reverse the effects of si-circ_0001821 transfection on the proliferation, migration, invasion and apoptosis of A431 cells. Conclusion: circ_0001821 regulates the proliferation, migration, invasion and apoptosis of CSCC A431 cells by targeting miR-203.
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Project Supported:
Project supported by the Natural Science Foundation of Hainan Province (No.814344)
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