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Archive > Volume 28 Issue 9 > 2021,28(9):900-907. DOI:10.3872/j.issn.1007-385X.2021.09.006 Prev Next
Basic Research
LINC01140 regulates the proliferation and invasion of esophageal squamous cell carcinoma Eca109 cells via miR-452-5p/Wnt/β-catenin axis
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Abstract:
Objective: To investigate the expression of lncRNA LINC01140 in esophageal squamous cell carcinoma (ESCC) tissues and cell lines, and to explore its effect on the proliferation and invasion of Eca109 cells as well as the possible molecular mechanism. Methods: The clinical data of 133 ESCC patients who were treated in the Fourth Hospital of Hebei Medical University from March 2012 to May 2015 and the data of 182 ESCC tissues and 286 normal esophageal mucosa tissues included in GEPIA database, as well as ESCC cell lines (Kyse150, Eca109, TE13) were collected for this study. The expression level of LINC01140 in ESCC tissues and cell lines was detected by qPCR method, and the relationship between its expression level and clinicopathological features as well as the prognosis of ESCC patients was further analyzed. pcDNA3.1-LINC01140, negative control (pcDNA3.1-NC) or miR-452-5p mimics and negative control (miR-NC) were respectively transfected into Eca109 cells. Then, MTS and Transwell assay were performed to assess the effect of LINC01140 on proliferation and invasion of transfected cells. The interaction between LINC01140 and miR-452-5p, as well as the effect of LINC01140 on the activation of Wnt/β-catenin pathway was detected by Dual-luciferase reporter gene assay and TOP/FOP reporter gene system. Results: The expression of LINC01140 was downregulated in ESCC tissues and cell lines (all P<0.01). Low expression of LINC01140 was closely correlated with the age, lymph node metastasis, TNM stage and the OS of ESCC patients (all P <0.01). Overexpression of LINC01140 significantly reduced the proliferation and invasion ability of Eca109 cells (all P<0.01). Mechanistic analysis indicated that LINC01140 affected the activation of Wnt/β-catenin signaling pathway possibly via competitively sponging miR-452-5p to further regulate the malignant biological behaviors of Eca109 cells. Conclusion: LINC01140 affects proliferation and invasion of ESCC cells via regulating miR-452-5p/Wnt/β-catenin axis. LINC01140 is expected to be a potential target for the targeted therapy and the molecular marker for the prognosis evaluation of ESCC patients.
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Project Supported:
Project supported by the Natural Science Foundation of Hebei Province (No. H2020206368), the Key Project of Medical Science Research in Hebei Province (No. 20210399), and the Talent Engineering Training Project of Hebei Province (No.201901035)
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