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Archive > Volume 28 Issue 9 > 2021,28(9):908-913. DOI:10.3872/j.issn.1007-385X.2021.09.007 Prev Next
Basic Research
lncRNA LINC01410 regulates the proliferation, apoptosis and temozolomide sensitivity of glioma A172 cells via miR-205-5p
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Abstract:
Objective: To investigate the effect of lncRNA LINC01410 on the proliferation, apoptosis and temozolomide (TMZ) sensitivity of glioma A172 cells and the underlying mechanism. Methods: qPCR method was used to determine the expression of LINC01410 in glioma cell lines (H4, SHG-44, A172) and normal astrocytes (HA1800). LINC01410 shRNA, shRNA control and miR-205-5p inhibitor and inhibitor control were respectively transfected into A172 cells, which were then treated with 400 μmol/L TMZ. Then, MTT assay and Flow cytometry were used to detect proliferation and apoptosis, and WB was used to detect protein expression of Bax, Bcl-2, cyclin D1 and p27 in transfected A172 cells. The target gene of LINC01410 was predicted by the online bioinformatic software LncBase, and the Dual-luciferase reporter gene system was used to verify the targeting relationship between LINC01410 and miR-205-5p. Results: The expression level of LINC01410 in three glioma cells was higher than that in normal astrocytes HA1800 cells (all P <0.01) with the highest expression level in A172 cells (P<0.01). After LINC01410 shRNA transfection and TMZ treatment, the proliferation decreased while the apoptosis rate increased in A172 cells, and the proportion of cells at G1 phase increased (all P<0.01); moreover, the protein expression level of Bax and p27 increased (all P<0.01), and the expression level of Bcl-2 and cyclin D1 decreased in A172 cells (all P<0.01). Dual-luciferase reporter gene assay verified that LINC01410 could targetedly bind with miR-205-5p, and down-regulation of LINC01410 promoted miR-205-5p expression. Transfection of miR-205-5p inhibitor could reverse the effects of down-regulation of LINC01410 and TMZ treatment on the proliferation, cell cycle and apoptosis of A172 cells. Conclusion: Down-regulation of lncRNA LINC01410 can inhibit proliferation, block cell cycle, induce cell apoptosis, and improve TMZ sensitivity of glioma A172 cells, the mechanism of which may be related with its targeted regulation of miR-205-5p.
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Project Supported:
Project supported by the Scientific Research Foundation of Health Bureau of Hebei Province (No. 20201493)
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