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Effect and mechanism of MLL5 knock-out on the growth of colon cancer CT26 cell transplanted tumor
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Abstract:
Objective: To investigate the effect of mixed lineage leukemia 5 (MLL5) gene on growth of colon cancer CT26 cell transplanted tumors in mice and its molecular mechanism. Methods: The colon cancer CT26 cell model with MLL5 gene deletion or double deletion of MLL5 and DDX58 (encoding RIG-1 gene) was constructed using CRISPR/Cas9 technology, and the knock-out efficiency was verified using Sanger sequencing and WB. Then, the constructed CT26 cells were inoculated into the scapular subcutaneous tissues of wild BALB/c mice and immunodeficient mice (NSG) to establish the gene-deficient CT26 cell transplanted tumor model. The tumor growth and the overall survival (OS) of tumor-bearing mice were observed. Results: In wild BALB/c mice, MLL5 gene depletion significantly slowed the tumor growth down and prolonged the OS of mice as compared with the mice transplanted with CT26 cells with normal MIL5 expression (P
Keywords:
colon cancer ; mixed lineage leukemia 5 (MLL5) ; retinoic acid inducible gene-1 (RIG-1) ; CT 26 cell ; tumor immunity ; CRISPR/Cas9 technology
