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Archive > Volume 29 Issue 3 > 2022,29(3):218-224. DOI:10.3872/j.issn.1007-385X.2022.03.008 Prev Next
Clinical Research
Clinical significance of LINC00997 expression in gastric cardia adenocarcinoma and its effect on migration, invasion and epithelial-mesenchymal transition of SGC7901 cells
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Abstract:
Objective: To detect the expression of LINC00997 in gastric cardia adenocarcinoma (GCA) tissues and gastric cancer cells and analyze its relationship with the clinicopathological features and prognosis of GCA patients, as well as to explore the effect of LINC00997 knockdown on the migration, invasion and epithelial-mesenchymal transition (EMT) of SGC7901 cells. Methods: Based on the TCGA and GTEx database, the expression of LINC00997 in gastric cancer tissues and its relationship with the prognosis of gastric cancer patients were analyzed. qPCR was applied to detect the expression of LINC00997 in 68 pairs of GCA tissues and the corresponding para-cancerous tissues and gastric cancer cells. The association between its expression level and clinicopathological characteristics and prognosis of patients was also analyzed. The effects of LINC00997 knockdown on migration and invasion of gastric cancer SGC7901 cells were examined by Wound-healing assay and Transwell invasion assay, respectively. The effects of LINC00997 knockdown on the expression of EMT-related markers E-cadherin, N-cadherin and vimentin were detected by qPCR and WB methods. Results: The expression of LINC00997 in gastric cancer tissues was significantly higher than that in para-cancerous tissues (P<0.05), and the overall survival (OS) rate and disease-free survival (DFS) rate of the LINC00997 high expression group was significantly lower than that of the low expression group (P<0.05 or P<0.01). The expression of LINC00997 in 68 GCA tissues was significantly higher than that in corresponding para-cancerous tissues (P<0.01), and was correlated with lymph node metastasis, TNM stage and OS of the patients (P<0.05 or P<0.01). The migration and invasion of SGC7901 cells with LINC00997 knockdown were significantly suppressed (all P<0.01), the expression levels of N-cadherin and vimentin were decreased while the expression of E-cadherin was increased (P<0.05 or P<0.01). Conclusion: LINC00997 is highly expressed in GCA tissues and cells. The high expression of LINC00997 may promote the migration, invasion and EMT process of GCA, and is expected to become a candidate molecular marker for prognostic evaluation of patients with GCA.
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Clc Number:
R735.2; R730.2; R730.7
