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Archive > Volume 29 Issue 6 > 2022,29(6):527-533. DOI:10.3872/j.issn.1007-385X.2022.06.003 Prev Next
Basic Research
circ_0000615 regulates proliferation, migration and invasion of cholangiocarcinoma cells by targeting miR-432-5p
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Abstract:
Objective: To investigate the expression of circular circ_0000615 in cholangiocarcinoma cells and its effect on the proliferation, migration and invasion ability of tumor cells as well as the possible regulatory mechanism. Methods: The expression level of circ_0000615 in cholangiocarcinoma cells (TFK-1, RBE, CCLP-1 and QBC939) and normal bile duct epithelium HIBEC cells was detected by qPCR. The targeting relationship between circ_0000615 and miR-432-5p was verified by the Dual-luciferase reporter gene assay. si-NC, si-circ_0000615 (si-circ-1, si-circ-2), inhibitor-NC and inhibitor-miR-432-5p were separately or jointly transfected into CCLP-1 and QBC939 cells, namely si-NC group, si-circ-1 group, si-circ-2 group, si-NC+inh-NC group, si-NC+inh-miR-432-5p group, and si-circ-1+inh-miR-432-5p group. The proliferation, migration and invasion of cholangiocarcinoma cells in each group were detected by CCK-8, EdU and Transwell experiments. Results: circ_0000615 was highly expressed while miR-432-5p was lowly expressed in cholangiocarcinoma tumor cells (P
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