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Archive > Volume 29 Issue 6 > 2022,29(6):534-540. DOI:10.3872/j.issn.1007-385X.2022.06.004 Prev Next
Basic Research
lncRNA SNHG14 regulates the malignant biological behaviors of thyroid cancer SW579 cells by targeting miR-433-3p
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Abstract:
Objective: To explore the effect of lncRNA SNHG14 on the malignant biological behaviors of thyroid cancer SW579 cells and its mechanism. Methods: The cancer tissue and paracancerous tissue samples of 20 patients with thyroid cancer admitted to Qinghai Provincial People's Hospital from October 2017 to December 2018 were collected. The expression of SNHG14 and miR-433-3p in thyroid carcinoma tissues and corresponding paracancerous tissues was determined by qPCR. Depending on the transfectant, SW579 cells were divided into the si-NC group (transfected with si-NC), si-SNHG14 group (transfected with si-SNHG14), miR-NC group (transfected with miR-NC), and miR-433-3p mimic group (transfected with miR-433-3p mimic), si-SNHG14+anti-miR-NC group (co-transfected with si-SNHG14 and anti-miR-NC), and si-SNHG14+anti-miR-433-3p group (co-transfected with si-SNHG14 and anti-miR-433-3p). MTT method, FCM, and Transwell test were used to detect the changes in proliferation, cell cycle, apoptosis rate, migration, and invasion ability of SW579 cells after transfection, respectively. The dual-luciferase reporter gene assay was employed to analyze whether SNHG14 could bind to miR-433- 3p, and the regulation relationship between SNHG14 and miR-433-3p was detected by qPCR. Results: Compared with the paracancerous tissues, the expression of SNHG14 in thyroid carcinoma tissues was significantly increased while the expression level of miR-433-3p was obviously decreased (all PObjective: To explore the effect of lncRNA SNHG14 on the malignant biological behaviors of thyroid cancer SW579 cells and its mechanism. Methods: The cancer tissue and paracancerous tissue samples of 20 patients with thyroid cancer admitted to Qinghai Provincial People's Hospital from October 2017 to December 2018 were collected. The expression of SNHG14 and miR-433-3p in thyroid carcinoma tissues and corresponding paracancerous tissues was determined by qPCR. Depending on the transfectant, SW579 cells were divided into the si-NC group (transfected with si-NC), si-SNHG14 group (transfected with si-SNHG14), miR-NC group (transfected with miR-NC),and miR-433-3p mimic group (transfected with miR-433-3p mimic), si-SNHG14+anti-miR-NC group (co-transfected with si-SNHG14 and anti-miR-NC), and si-SNHG14+anti-miR-433-3p group (co-transfected with si-SNHG14 and anti-miR-433-3p). MTT method, FCM,and Transwell test were used to detect the changes in proliferation, cell cycle, apoptosis rate, migration, and invasion ability of SW579 cells after transfection, respectively. The dual-luciferase reporter gene assay was employed to analyze whether SNHG14 could bind to miR-433-3p, and the regulation relationship between SNHG14 and miR-433-3p was detected by qPCR. Results: Compared with the paracancerous tissues, the expression of SNHG14 in thyroid carcinoma tissues was significantly increased while the expression level of miR-433-3p was obviously decreased (all P<0.05). SNHG14 inhibition or miR-433-3p overexpression could reduce cell proliferation, the number of migrated and invaded cells (P<0.05), increase the rate of apoptosis (P<0.05) and the proportion of cells in the G1 phase (P<0.05), and decrease the proportion of cells in the S phase (P<0.05). Dual-luciferase reporter gene assay demonstrated that SNHG14 could bind to miR-433-3p, and inhibition of SNHG14 expression could promote the expression of miR-433-3p in SW579 cells (all P<0.05). Simultaneous inhibition of miR-433-3p expression partialy reversed the inhibitory effect of SNHG14 downregulation on cell proliferation, apoptosis, migration, and invasion of SW579 cells (all P<0.05). Conclusion: lncRNA SNHG14 is highly expressed while miR-433-3p is lowly expressed in thyroid cancer tissues. lncRNA SNHG14 can promote the proliferation, migration and invasion and inhibit apoptosis of thyroid cancer SW579 cells by targeted binding to miR-433-3p.
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