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Archive > Volume 29 Issue 7 > 2022,29(7):631-638. DOI:10.3872/j.issn.1007-385X.2022.07.004 Prev Next
Basic Research
Molecular mechanisms of sorafenib resistance induced by lncRNA SUMO1P3 in hepatocellular carcinoma HepG2 cells
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Abstract:
Objective:Toinvestigatethemolecular mechanisms of sorafenib (SR) resistance induced by long non-coding RNA small ubiquitinlike modifier 1 pseudogene 3 (lncRNA SUMO1P3) in hepatocellular carcinoma (HCC). Methods: HCC cells HepG2 were cultured in vitro and acted as the control group. The expression of SUMO1P3 was detected by qPCR in HepG2/SR cells, which was induced by continuously increasing the contact concentration. si-SUMO1P3 and si-NC were transfected into HepG2/SR cells by Lipofectamine. pc-SUMO1P3 and pc-DNA were transfected into HepG2 cells respectively, and then treated with 5 μmol/L SR for 24 hours. qPCR was used to detect the expression levels of SUMO1P3 in the transfected cells. The proliferation, migration, invasion and apoptosis of transfected cells were detected by CCK-8, Transwell assay, and FCM, respectively. Western blotting assay was conducted to detect the expression of cyclin D1,Bcl2,BAX,MMP-2, and MMP-9 in the different cell groups. Results: Sorafenib-resistant HepG2/SR cells were successfully constructed.The expression level of SUMO1P3 in the HepG2/SR cells was significantly higher than that in the HepG2 cells(P<0.01).After SUMO1P3 was knocked outintheHepG2/SRcells, comparedwithinthe si-NC group,inthe si-SUMO1P3 group the expression of SUMO1P3, the proliferation, migration and invasion of cells and the protein levels of cyclin D1, Bcl2, MMP-2 and MMP-9 were all significantly lower while the percentage of apoptotic cells and the expression of BAX were significantly higher (P<0.05 or P<0.01). After SUMO1P3 was over-expressed in the HepG2 cells, compared with in the pc-DNA group, the proliferation, migration and invasion of cells in the pc-SUMO1P3 group and the protein levels of cyclin D1, Bcl2, MMP-2 and MMP-9 were all significantly higher while the percentage of apoptotic cells and the expression of BAX were significantly lower (P<0.05 or P<0.01). Similarly, compared with in the pc-DNA+SR group, the proliferation, migration and invasion of HepG2 cells in the pc-SUMO1P3+SR group and the protein levels of cyclin D1, Bcl2, MMP-2 and MMP-9 were all significantly higher while the percentage of apoptotic cells and the expression of BAX were significantly lower (P<0.05 or P<0.01). Conclusion: lncRNA SUMO1P3 can induce the resistance of HepG2 cells to sorafenib by promoting the proliferation, migration and invasion and inhibiting the apoptosis of HepG2 cells.
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