Mobile website
Archive > Volume 29 Issue 7 > 2022,29(7):659-664. DOI:10.3872/j.issn.1007-385X.2022.07.008 Prev Next
Technigue and Method
A preparation method for the organoid model of patient-derived glioblastoma
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To explore a preparation method for glioblastoma organoid (GBO) derived from patients with glioblastoma (GBM). Methods: The fresh tumor tissue samples of eight GBM patients newly diagnosed and pathologically confirmed in Guangdong 999 Brain Hospital in 2021 were selected, cut into tissue fragments of 0.5-1 mm in size and cultured in a special medium. When the tissue fragments grew into a spherical shape and the diameter reached 1 mm, they were cut into small pieces and passaged. GBOs cultured for more than 2 weeks were selected for paraffin-embedding, sectioned, and then stained with H-E and immunohistochemical staining. Histological and cytological comparisons were performed with parental GBM tissues. Results: Two cases of GBO that could be passaged and cryopreserved were successfully prepared and a GBO biobank was established. The results of H-E staining showed that GBOs retained tissue structure and cell morphology similar to those of the parental GBM tissues. The results of immunohistochemical analysis showed that the expressions of GFAP, OLIG2, Ki67 and ATRX in GBO and parental GBM tissues were basically consistent. Conclusion: GBO can be generated in vitro by trimming patient-derived GBM tissue and culturing it in a suitable medium. The GBO prepared by this method is consistent with the parental GBM tissue at the histological and cytological levels.
Keywords:
Project Supported:
Clc Number:
