Mobile website
Archive > Volume 29 Issue 8 > 2022,29(8):724-731. DOI:10.3872/j.issn.1007-385X.2022.08.004 Prev Next
Basic Research
TRIM59 regulates malignant biological behaviors of skin cutaneous melanoma cells SK-MEL-2 through combination with BCLAF1
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To explore the mechanism of tripartite motif-containing 59 (TRIM59) regulating the proliferation, cell cycle,apoptosis, migration and invasion of human skin melanoma cells SK-MEL-2, and its relationship with Bcl2-associated transcription factor (BCLAF1). Methods: qPCR and WB assay were used to measure the mRNA and protein expression of TRIM59 in human epidermal melanocytes HEMN-LP, human skin melanoma cells SK-MEL-2, UACC903, A375, and 36 cases of human skin melanoma tissues collected from February 2019 to July 2021 in Xingtai people's Hospital. Si-con and si-TRIM59 were transfected into SK-MEL-2 cells using liposomes. WB assay was used to detect the effects of interference with the expression of TRIM59 on cyclin D1 (CCND1),cyclin-dependent kinase 2 (CDK2), tumor suppressor protein gene (TP53) and BCLAF1 protein expression. CCK-8 assay, flow cytometry, scratch test and Transwell test were used to detect cell activity, apoptosis, migration and invasion. The binding ability of TRIM59 protein and BCLAF1 was detected by Co-IP assay. Results: Compared with the HEMN-LP group, the mRNA and protein expression of TRIM59 BCLAF1 protein in SK-MEL-2, UACC903and A375 cells were significantly increased (P<0.05). The expression level of TRIM59 in SK-MEL-2 cells were the highest. Compared with the si-con group and the Normal group, after silencing TRIM59,the activity of SK-MEL-2 cells was significantly decreased; the G2 phase of the cell cycle was blocked; the protein expressions of CCND1 and CDK2 were decreased significantly; the TP53 protein and apoptosis rate were significantly increased; the scratch inhibition rate was significantly increased, and the numbers of migration and invasion cells were significantly decreased. (all P<0.05).The results of co-immunoprecipitation experiments showed that there was a protein-binding relationship between TRIM59 and BCLAF1. There was a significant positive correlation between TRIM59 and BCLAF1 expression in tumor tissues (r=0.878, P<0.001).Conclusion: Silencing TRIM59 expression could inhibit the proliferation, migration and invasion of skin cutaneous melanoma cells,promote apoptosis and inhibit the malignant biological behaviors of SK-MEL-2 cells. The mechanism may be related to the binding of TRIM59 with BCLAF1.
Keywords:
Project Supported:
Clc Number:
