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Archive > Volume 29 Issue 9 > 2022,29(9):806-812. DOI:10.3872/j.issn.1007-385X.2022.09.004 Prev Next
Basic Research
Ethanol extract from Euphorbia humifusa inhibits the malignant biological behaviors of colorectal cancer SW480 cells by regulating the circRHOT1/miR-29a-3p molecular axis
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Abstract:
Objective: To explore the effect of ethanol extract from euphorbia humifusa (EEEH) on the biological behaviors of human colorectal cancer SW480 cells and its molecular mechanism. Methods: Human colorectal cancer SW480 cells were cultured in vitro,divided into the following experimental groups: Con group, EEEH-L group, EEEH-M group, EEEH-H group,si-NC group, si-circRHOT1 group, EEEH-H+pcDNA group and EEEH-H+pcDNA-circRHOT1 group and transfected with si-NC, si-circRHOT1, pcDNA and PCDNA-circRHOT1 respectively. CCK-8 assay, cell clone formation assay, Transwell assay were used to detect cell proliferation,migration and invasion ability in each group after transfection. qPCR method was employed to detect the expression of circRHOT1 and miR-29a-3p in all the groups of SW480 cells after transfection, and the expressions of MMP-2 and MMP-9 proteins were detected by WB. The dual luciferase reporter experiment was used to detect the targeting relationship between circRHOT1 and miR-29a-3p.Results: Compared with the Con group, the protein expressions of MMP-2 and MMP-9 in SW480 cells in EEEH-L, EEEH-M, and EEEH-H groups were significantly decreased (all P<0.05), the expression of circRHOT1 decreased (all P<0.05) and the expression of miR-29a-3p increased (all P<0.05) in a dose-dependent manner. The cell survival rate, the number of cell clone formation and the number of migration and in asive cells were decreased (all P<0.05). circRHOT1 can target and negatively regulate the expression of miR-29a-3p. Knockdown of circRHOT1 can inhibit the proliferation, migration and invasion of SW480 cells, while overexpression of circRHOT1 can attenuate the inhibitory effects of EEEH on the proliferation, migration and invasion of SW480 cells.Conclusion: EEEH can inhibit the proliferation, migration and invasion of colorectal cancer SW480 cells by regulating the circRHOT1/miR-29a-3p axis.
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