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Archive > Volume 29 Issue 9 > 2022,29(9):813-821. DOI:10.3872/j.issn.1007-385X.2022.09.005 Prev Next
Clinical Research
Expression and clinical significance of E3 ubiquitin ligase HECW2 in gastric adenocarcinoma tissues
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Abstract:
Objective: To analyze the expression and clinical significance of E3 ubiquitin ligase (HECW2) in gastric adenocarcinoma using a bioinformatics approach to provide new clues for finding diagnostic and prognostic biomarkers for gastric adenocarcinoma.Methods: The expression of HECW2 in pan-cancer and its relationship with the prognosis of pan-cancer were analyzed by R language.The expression of HECW2 in gastric adenocarcinoma tissues and its relationship with clinicopathological features were analyzed with the help of UCSC Xena, HPA, and Kaplan-Meier Plotter databases. The WB method was used to detect HECW2 protein levels in Chinese gastric adenocarcinoma tissues and their paraneoplastic tissues to verify the analysis results in the database. The relationship between HECW2 and immune infiltration of gastric adenocarcinoma was analyzed with the help of TIMER and Cibersort databases.GO functional analysis and KEGG signaling pathway enrichment analysis and correlation gene analysis were performed for HECW2 in gastric adenocarcinoma by LinkedOmics database. Results: The results of raw signal analysis showed that HECW2 was significantly highly expressed in 12 tumors including gastric adenocarcinoma among 33 different types of tumors (all P<0.05), and the results of WB method showed that HECW2 was also significantly highly expressed in Chinese gastric adenocarcinoma tissues (P<0.05). Higher HECW2 expression level was associated with shorter OS and higher abundance of infiltration of immune cells including CD4+T cells, macrophages, neutrophils and dendritic cells in gastric adenocarcinoma patients (all P?0.01). In addition, HECW2-associated signaling pathways were mainly enriched in extracellular matrix receptor interactions, adherent spots, cell adhesion and oxidative phosphorylation (all P?0.01). Conclusion: HECW2 is significantly overexpressed in gastric adenocarcinoma tissues and is closely related to the poor prognosis and immune invasion of gastric adenocarcinoma, and has great potential as a prognostic biomarker and target therapy for gastric adenocarcinoma.
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