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Archive > Volume 29 Issue 10 > 2022,29(10):879-888. DOI:10.3872/j.issn.1007-385X.2022.10.003 Prev Next
Basic Research
miR-192-5p regulates the malignant biological behaviors of pancreatic cancer PANC-1 cells by targeting ZEB2
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Abstract:
Objective: To investigate the effect and mechanism of miR-192-5p targeting ZEB2 (zinc finger E-box binding homeobox 2)on the proliferation, migration, invasion and EMT (epithelial-mesenchymal transition) of pancreatic cancer PANC-1 cells. Methods:The TCGA database was used to analyze the expression of miR-192-5p and ZEB2 as well as their correlation in pancreatic cancer tissue.The expression levels of miR-192-5p and ZEB2 were detected by qPCR and WB in human normal pancreatic epithelial HPNE cells and pancreatic cancer PANC-1 cells. PANC-1 cells were transfected with liposome, and divided into miR-192-5p mimic group, Mimic NC group, miR-192-5p inhibitor group, Inhibitor NC group, Mimic NC+pcDNA3.1 group, miR-192-5p mimic+pcDNA3.1 group, andmiR-192-5p mimic+pcDNA3.1-ZEB2 group. The proliferation, colony formation, migration and invasion abilities of transfected PANC-1 cells were detected by CCK-8, colony formation, scratch healing and Transwell experiments, respectively. The expression of ZEB2,E-cadherin and vimentin was detected by qPCR, WB method and double immunofluorescence assay. Bioinformatics was used to predict the target gene of miR-192-5p, and the regulatory effect of miR-192-5p on the target gene was verified by dual luciferase report gene experiment. Results: The expression of ZEB2 in pancreatic cancer tissue was significantly higher than that in normal pancreatic tissue (P<0.01), and the expression of miR-192-5p and ZEB2 in pancreatic cancer tissue was inversely correlated (r=-0.419, P<0.01).Compared with normal pancreatic epithelial HPNE cells, the expression level of miR-192-5p was significantly lower while the expression level of ZEB2 protein was higher in pancreatic cancer PANC-1 cells (all P<0.01). Overexpression of miR-192-5p significantly reduced the proliferation, colony formation, migration and invasion ability of PANC-1 cells, upregulated the mRNA and protein expression of E-cadherin, and downregulated the mRNA and protein expression of vimentin and ZEB2, while inhibition of miR-192-5p achieved the opposite results (P<0.05 or P<0.01). miR-192-5p targetedly regulated the expression of ZEB2, and overexpression of ZEB2 could reverse the inhibitory effects of miR-192-5p overexpression on the proliferation, colony formation,migration, invasion and EMT of PANC-1 cells. Conclusion: miR-192-5p regulates the malignant biological behaviors of pancreatic cancer PANC-1 cells by targeting ZEB2.
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