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Archive > Volume 29 Issue 10 > 2022,29(10):889-895. DOI:10.3872/j.issn.1007-385X.2022.10.004 Prev Next
Basic Research
miR-515-5p effects the carcinogenesis of esophageal cancer via targeting HDAC2 and its molecular mechanism
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Abstract:
Objective: To investigate the effects of miR-515-5p on proliferation, migration and invasion of esophageal cancer (EC) cells and the molecular mechanism. Methods: Cancer tissue specimens from 60 patients with esophageal cancer that surgically resected at the Fourth Hospital of Hebei Medical University from June 2020 to December 2020, esophageal epithelial tissue specimens from 20 healthy adults, as well as esophageal cancer cell lines (TE1, Eca109, KYSE30 and KYSE170) were selected for this study. Quantitative reverse transcription polymerase chain reaction (qPCR) was performed to detect miR-515-5p expression in above mentioned EC tissues and cells. Eca109 cells were transfected with miR-515-5p mimics or its negative controls, and TE1 cells were transfected with miR-515-5p inhibitors or its negative controls. The transfection efficiency was detected by qPCR, and the proliferation, migration and invasion of transfected cells were detected by CCK-8 assay and transwell assay respectively. Bioinformatics tools were used to predict the downstream target genes of miR-515-5p, and HDAC2 (histone deacetylase 2) was verified to be a target gene of miR-515-5p by dual luciferase reporter gene assay. GEPIA and TCGA databases were used to analyze the expression of HDAC2 in EC tissues and its correlation with clinical characteristics of EC patients. Results: miR-515-5p was downregulated in EC tissues and cells (all P<0.01).Over-expression of miR-515-5p inhibited the proliferation, migration and invasion ability of Eca109 cells (P<0.05 or P<0.01), while down-regulation of miR-515-5p enhanced the proliferation, migration and invasion ability of TE1 cells (both P<0.05). Furthermore,HDAC2 was demonstrated to be a target gene of miR-515-5p by dual luciferase reporter gene assay. qPCR and Western blotting results showed that miR-515-5p negatively regulated the mRNA and protein expression of HDAC2. Rescue experiments confirmed that miR-515-5p inhibited the proliferation, migration and invasion ability of esophageal cancer Eca109 cells by targeting HDAC2 (P<0.05 orP<0.01). Conclusion: miR-515-5p affects proliferation, migration and invasion of EC cells by targeting HDAC2.
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